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Each lot of this antibody has been quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The antibody has been tested for Western blotting of mouse and human β-catenin protein.
Whole cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-beta-catenin antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
β -catenin is a 94 kD nuclear and cytoplasmic protein containing Armadillo repeats. This protein is involved in cell-cell adhesion and the wingless/WNT signaling pathway, and has been implicated as an oncogene. β-catenin can be modified by glycosylation, phosphorylation, and ubiquitination to target it for degradation. This protein has been shown to interact with E-cadherin, α-catenin, axin, APC, GSK-3β, and Tcf/Lef. Dissociation from axin allows cytoplasmic accumulation. After nuclear translocation, it binds Tcf/Lef.
Degraded through phosphorylation then ubiquitination, disassociation from axin complex allows cytoplasmic accumulation. Translocation to nucleus, binds Tcf/Lef
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Purified anti-β-catenin
Whole cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-beta-catenin antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
