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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a starting dilution of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
MOLT-4 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal anti-ß-catenin antibody.
Immunofluorescent microscope analysis of Hela cells using anti β-catenin polyclonal antibody (red). Nuclei were stain with DAPI (blue).
β-catenin is a 94 kD nuclear and cytoplasmic protein containing Armadillo repeats. This protein is involved in cell-cell adhesion, wingless/WNT signaling pathway, and has been implicated as an oncogene. β-catenin can be modified by glycosylation, phosphorylation, and ubiquitination to target for degradation. This protein has been shown to interact with E-cadherin, α-catenin, axin, APC, GSK-3β, and Tcf/Lef. Dissociation from axin allows cytoplasmic accumulation, after nuclear translocation binds Tcf/Lef. The Poly6001 antibody has been reported to be useful for Western blotting and immunofluorescence of the mouse and human β-catenin protein.
Degraded through phosphorylation then ubiquitination, disassociation from axin complex allows cytoplasmic accumulation. Translocation to nucleus, binds Tcf/Lef
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-β-catenin
MOLT-4 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal anti-ß-catenin antibody.
Immunofluorescent microscope analysis of Hela cells using anti β-catenin polyclonal antibody (red). Nuclei were stain with DAPI (blue).
