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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Huttenrauch F, et al. 2005. J. Biol. Chem. 280:37503.
NIH/3T3 (lane 1) and Hela (lane 2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with β-Arrestin-1 (25G10). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
The arrestin family consists of four members. Visual arrestins (arrestin 1 and 4) are exclusively expressed in the retina. Beta-arrestin 1 and Beta- arrestin 2, also known as arrestin 2 and arrestin 3, are ubiquitously expressed in most tissues. They are negative regulators of G-protein-coupled receptor (GPCR) signaling. Upon GPCR activation, beta-arrestins trans-locate to the cell membrane and bind to the agonist-occupied receptors and ultimately resulting the receptor internalization and desensitization. Recent evidence suggest that they also function as scaffold proteins to interact with several cytoplasmic proteins and link GPCRs to intracellular signaling pathways.
Other Names:
Arrestin 2, beta-arrestin-1
Structure:
The two beta-arrestin isoforms share 78% amino acid sequences. Most of the coding difference appear in the C-termini. MW about 50 kd.
Distribution:
Expressed ubiquitously in all cells and tissues.
Function:
Function as negative regulators of GPCR
Antigen References:
1. Parruti GP, et al. 1993. J. Biol. Chem. 268:9753. 2. DeWire SM, et al. 2007. Ann. Rev Physiol. 69:483.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-β-Arrestin-1
NIH/3T3 (lane 1) and Hela (lane 2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with β-Arrestin-1 (25G10). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
