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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. For IHC, use a 1:100 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Lawson BR, et al. 2007. J. Immunol. 178:5366. 2. Joyce CW. 2006. J. Biol. Chem. 281:33053. 3. Yanagiya T,et al.2007.Obesity.15:572.PubMed 4. KishidaT,et al.2007.J. Immunol. 179:8554.PubMed 5. Ouimet M, et al. 2008. Arterisocler Thromb Vasc Biol. 28:1144. PubMed 6. Toltlt LJ,et al. 2008.J. Immunol. 181:2165. PubMed 7. Sawada. T,et al. 2008. J. Biol. Chem. 283:26820. PubMed 8. Ikeda D, et al. 2008. Endocrinology. 149:6037. PubMed 9. Rahman MK, et al. 2010. J. Immunol. 184:7247. PubMed 10. Shikama Y, et al. 2011. Innate Immun. 17:3. PubMed 11. Zhang Y, et al. 2012. Am J Physiol Renal Physiol. 302:70. PubMed.
Hela cell extract was western blotted with Poly6221. Proteins were visualized using a HRP donkey anti-rabbit antibody and a chemiluminescence detection system.
Immunofluorescent microscope analysis of Hela cells using Poly6221, followed by Rhodamine Red-X goat anti-rabbit IgG and DAPI.
Formalin-fixed paraffin-embedded human kidney tissue was stained with Poly6221 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
β-actin is a ubiquitously expressed and highly conserved 42 kD cytoplasmic protein involved in cell motility. This critical cytoskeletal component can be disrupted by drugs such as cytochalasin. Because β-actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection (such as Western blotting). The Poly6221 antibody has been shown to be useful for Western blotting of mouse, rat, and human β-actin.
Other Names:
Actin, cytoplasmic 1
Structure:
Member of the actin family; 42 KD
Distribution:
Ubiquitously expressed in the cytoplasm of all eukaryotic cells
Function:
Actins are highly conserved proteins that are involved in cell motility
Regulation:
Three actin isoforms exist in vertebrates; alpha, found in muscle tissues; beta and gamma, found as cytoskeletal components and mediators of internal cell motility. Drugs such as cytochalasin disrupt the actin network.
Interaction:
Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) that forms a two-stranded helix. Each actin can bind to four others.
Antigen References:
1. Hanukoglu I, et al. 1983. J. Mol. Biol. 163:673. 2. Nakajima-Iijima S, et al. 1985. Proc. Natl. Acad. Sci. 82:6133. 3. Ponte P, et al. 1984. Nucleic Acids Res. 12:1687.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-β-actin
Hela cell extract was western blotted with Poly6221. Proteins were visualized using a HRP donkey anti-rabbit antibody and a chemiluminescence detection system.
Immunofluorescent microscope analysis of Hela cells using Poly6221, followed by Rhodamine Red-X goat anti-rabbit IgG and DAPI.
Formalin-fixed paraffin-embedded human kidney tissue was stained with Poly6221 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
