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Each lot of this antibody is quality control tested by Western blotting and immunofluorescence microscopy. Western blotting, suggested working dilution(s): Use 0.5 µg antibody per ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution of 1:200. The optimal concentration should be determined by titration for each individual assay of interest.
COA:
Application References:
1. Tomassy GS, et al. 2010. P. Natl. Acad. Sci. USA 107:3576. 2. Cherrier T, et al. 2009. Oncogene 28:3380.
Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-CTIP2 antibody (clone 25B6). Proteins were visualized using a goat anti-rat-IgG secodary conjugated to HRP and chemiluminescence detection.
Jurkat cells were stained with anti-bcl11b, clone 25B6 (1:200 dilution), and tubulin-α, clone TU-01, followed by DyLight™ 549 conjugated anti-rat IgG and DyLight™ 488 conjugated anti-mouse IgG. Nuclei are stained with DAPI.
The transcription factor Bcl11b is expressed in thymus, mainly T cells. It is indispensable for T lineage development. When Bcl11b was deleted, T cells from all developmental stages acquired NK cell properties and concomitantly lost or decreased T cell-associated gene expression. These induced T-to-natural killer (ITNK) cells, which were morphologically and genetically similar to conventional NK cells, killed tumor cells in vitro, and effectively prevented tumor metastasis in vivo. Therefore, ITNKs may represent a new cell source for cell-based therapies.
894 amino acids with molecular weight of 96 kD. It encodes a C2H2-type zinc finger protein and is closely related to BCL11A.
Distribution:
Nucleus
Function:
Tumor-suppressor protein involved in T-cell lymphomas. A key regulator of both differentiation and survival during thymocyte development.
Interaction:
Interacts with TFCOUP1, SIRT1, ARP1, and EAR2
Antigen References:
1. Liu P, et al. 2011. Immunol. Rev. 238:138. 2. Ikawa T, et al. 2010. Science 329:93. 3. Li L, et al. 2010. Science 329:89. 4. Li P, et al. 2010. Science 329:85. 5. Tydell CC, et al. 2007. J. Immunol. 179:421. 6. Avram D, et al. 2002. Biochem. J. 368:555.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Bcl11b
Jurkat cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-CTIP2 antibody (clone 25B6). Proteins were visualized using a goat anti-rat-IgG secodary conjugated to HRP and chemiluminescence detection.
Jurkat cells were stained with anti-bcl11b, clone 25B6 (1:200 dilution), and tubulin-α, clone TU-01, followed by DyLight™ 549 conjugated anti-rat IgG and DyLight™ 488 conjugated anti-mouse IgG. Nuclei are stained with DAPI.
