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Purified Anti-Bcl10 Antibody
Material Safety Data Sheet (MSDS) Purified Anti-Bcl10 Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-Bcl10 Antibody
640701 25 µg $60.00     
640702 100 µg $110.00     
Clone: 151
Isotype: Mouse IgG1
Reactivity: Human
Immunogen: The epitope recognized by the antibody resides within amino acids 122-168 of human Bcl-10 molecule.
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation: The antibody was purified by protein G affinity chromatography
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store at 4°C.
Application: WB1 - Quality tested
ELISA, IHC(P)1,2 - These applications have been reported in the literature.
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application
References:
1. Ye, H. et al. 2000. Am. J. Pathol. 157:1147.
2. Ye, H. et al. 2003. Blood. 102:1012.
Western blot analysis of extracts

Western blot analysis of extracts from Jurkat cells, using anti-Bcl10, clone 151.


Description: BCL10 promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK.  It may be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex.  A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma), translocation t(1;14)(p22;q32). Defects in BCL10 are involved in various types of cancer.
Other Names: B-cell CLL/lymphoma 10, CIPER, CLAP
Structure: Contains 1 CARD domain. Predicted MW, 26 kD.
Distribution: Cytoplasm › perinuclear region
Function: Bcl10 promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK.
Regulation: Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
Interaction: MALT1, TRAF2, Caspase-9, BIRC2/c-IAP2
Antigen
References:
1. Willis, T.G. et al. 1999. Cell 96:35.
2. Zhang, Q. et al. 1999. Nat. Genet. 22:63.
3. Yui, D. et al. 2001. Oncogene 20:4317.
HRP Goat anti-mouse IgG (minimal x-reactivity)Poly4053ELISA,IHC,WB