Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.
Western blot analysis of extracts from Hela cells (lane 1) and human PBMC (lane 2), using anti-Bcl-XS/L, clone 2H12.
Bcl-X is one of the members of Bcl-2 family proteins, and exists in several isoforms generated by alternative splicing and plays a critical role in the control of apoptosis. The large isoform Bcl-XL (30KD)contains the highly conserved BH1 and BH2 amino acid domains and protects cells against apoptosis, whereas the shortisoform, Bcl-XS(18KD) which deletes the conserved BH1/BH2 domain by alternative mRNA splicing, promotes apoptosisby interactingwith Bcl-XL and Bcl-2.Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.Clone 2H12 has been shown to be useful for western blotting and immunoprecipitation, immunohistochemistry, and immunofluorescence of the Human, Mouse and Rat Bcl-XL and Bcl-XS proteins.
Other Names:
Apoptosis regulator Bcl-X, Bcl-2-like 1 protein
Structure:
Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. 5 named isoforms produced by alternative splicing: Bcl-X(S), Bcl-X(L), Bcl-X (beta), Bcl-X(gamma),and Bcl-X(δTM).
Distribution:
Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
Function:
Bcl-X plays a critical role in the control of apoptosis. The large isoform Bcl-XL (30KD) contains the highly conserved BH1 and BH2 amino acid domains and protects cells against apoptosis, whereas the short isoform, Bcl-XS (18KD) which deletes the conserve
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Bcl-XS/L
Western blot analysis of extracts from Hela cells (lane 1) and human PBMC (lane 2), using anti-Bcl-XS/L, clone 2H12.
