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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
This clone is also known as GI191E/A8.
Application References:
1. García JF, et al. 2006. J. Histochem Cytochem. 54:31.
Raji (lane 1) and A20 (lane 2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Bcl -6 monoclonal antibody, IG191E/A8. Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescent system.
Bcl-6 is a transcriptional regulator gene which codes for a nuclear zinc finger protein of 706 amino acids. Bcl-6 is expressed in T and B cells in germinal centers of lymphoid organs. It is also expressed in follicular lymphoma, Burkitt's lymphoma and majority of the Reed-Sternberg cells in Hodgkin's disease. Although Bcl-6 expression has been extensively studied in normal and neoplastic B cell development, subsets of follicular CD4+ T cells are also found to express Bcl-6.
Other Names:
BCL6
Structure:
A nuclear zinc finger protein with 706 amino acids, predicted Mw of around 80kD.
Distribution:
Nucleus, expressed in germinal center T and B cells.
Function:
Required for germinal center formation and antibody affinity maturation. May play a role in lymphoma and leukemia genesis.
Antigen References:
1. Ye BH, et al. 1993. Science 262:747. 2. Baron BW, et al. 1993. P. Natl. Acad. Sci. USA 90:5262. 3. Onizuka T, et al. 1995. Blood 86:28.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Bcl-6
Raji (lane 1) and A20 (lane 2) cell extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with anti- Bcl -6 monoclonal antibody, IG191E/A8. Proteins were visualized using goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescent system.
