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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1~4 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Hsu YT, et al. 1997. J. Biol. Chem. 272:13829. 2. Zuber J, et al. 2011. Genes Dev. 25:1628. PubMed.
Western blot analysis of extracts from mouse splenocytes using anti-Bcl-2, clone BCL/10C4.
Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist α=25 kD; β=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, intracellular membrane nuclear envelope, and endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Bcl-2 is modified by phosphorylation and ubiquitination. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.
Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
Regulation:
Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
Modification:
Phosphorylation, Ubiquitination
Antigen References:
1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214. 2. Yang E, et al. 1995. Cell 80:285. 3. Huang Z, et al. 2000. Oncogene 19:6627. 4. Deng X, et al. 2003. Blood. 102:3179.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-Bcl-2
Western blot analysis of extracts from mouse splenocytes using anti-Bcl-2, clone BCL/10C4.
FITC anti-Bcl-2
C57BL/6 splenocytes intracellular stained with BCL/10C4 FITC (cells were treated with BioLegend’s Foxp3 Fix/Perm buffer, cat# 421401 and then permeabilized with BioLegend’s Permeabilization Wash Buffer, cat# 421002)
Alexa Fluor® 488 anti-Bcl-2
C57BL/6 splenocytes intracellular stained with BCL/10C4 Alexa Fluor® 488 (cells were treated with BioLegend’s Foxp3 Fix/Perm buffer, cat# 421401 and then permeabilized with BioLegend’s Permeabilization Wash Buffer, cat# 421002)
PE anti-Bcl-2
C57BL/6 splenocytes intracellularly stained with BCL/10C4 PE (cells were treated with BioLegend’s Foxp3 Fix/Perm buffer, cat# 421401 and then permeabilized with BioLegend’s Permeabilization Wash Buffer, cat# 421002)
Alexa Fluor® 647 anti-Bcl-2
C57BL/6 splenocytes intracellularly stained with BCL/10C4 Alexa Fluor® 647 (cells were treated with BioLegend’s Foxp3 Fix/Perm buffer, cat# 421401 and then permeabilized with BioLegend’s Permeabilization Wash Buffer, cat# 421002)
