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Purified Anti-Bcl-2 Antibody
Material Safety Data Sheet (MSDS) Purified Anti-Bcl-2 Antibody     Product Data Sheet (HTML)     Product Data Sheet (PDF)    
Purified Anti-Bcl-2 Antibody
611901 50 µl (5 Western blots) $75.00     
611902 200 µl (20 Western blots) $225.00     
Clone: Poly6119
Isotype: Rabbit IgG
Reactivity: Human, Mouse, Rat
Immunogen: Peptide-KLH, N-terminal
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by antigen-affinity chromatography.
Storage & Handling: Upon receipt, store at 4°C.
Application: WB, IF
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.
Application
References:
1. Kim, B. E., et al., 2004. Exp Mol Med. 36: 444.
Hela cell extract was resolved

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Bcl-2 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. This antibody recognizes both the a and ß Bcl-2 isoforms.
Immunofluorescent microscope analysis of Hela

Immunofluorescent microscope analysis of Hela cells using anti-Bcl-2 polyclonal antibody, followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI.


Description: Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist α=25 kD; β=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, intracellular membrane nuclear envelope, and endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Bcl-2 is modified by phosphorylation and ubiquitination. The Poly6119 antibody has been shown to be useful for Western blotting of the human, mouse, and rat Bcl-2 protein.
Other Names: B-cell leukemia 2
Structure: Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD
Distribution: Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum
Function: Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
Regulation: Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
Modification: Phosphorylation, Ubiquitination
Interaction: Apaf-1, Raf-1, TP53BP2, caspase-3, heterodimers with Bax, Bad, Bak, Bcl-XL, Bag-1
Antigen
References:
1. Tsujimoto, Y., et al., 1986 PNAS 83:5214.
2. Yang, E., et al., 1995. Cell 80:285.
3. Huang, Z., et al., 2000. Oncogene 19:6627.
4. Deng, X., et al., 2003. Blood. 102:3179.
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