This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation:
This antibody was purified by affinity chromatography.
Concentration:
0.5 mg/ml
Storage & Handling:
Upon receipt, store undiluted at 4°C.
Application:
WB - Quality tested. IP*, IHC* - *This application has been reported in the literature.
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 μg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. Hsu YT., et al. 1997. J. Biol. Chem. 272: 13829.
Western blot analysis of extracts from Hela cells (lane 1) and human PBMCs (lane 2) using anti-Bax, clone 2D2.
Description:
Bax is a 21 kD pro-apoptotic protein known to regulate apoptosis. Bax is found in the cytoplasm, mitochondria, and nucleus and is highly expressed in hematopoietic stem cells, the ovary, and in the lymph node. Bax binds the anti-apoptotic protein Bcl-2 as a heterodimer or forms homodimers. The relative levels of pro-apoptotic proteins such as Bax and anti-apoptotic proteins such as Bcl-2 determines whether cell death will occur following an apoptotic stimulus. Bax accelerates the opening of mitochondrial VDAC altering membrane potential and allowing cytochrome c to pass out of the mitochondria into the cytosol to initiate downstream caspase activation. p53 can transcriptionally activate the Bax gene to induce apoptosis. Bax has been shown to be mutated in some human cancers. Clone 2D2 has been shown to be useful for western blotting, immunoprecipitation, immunofluorescence and immunohistochemistry of the human Bax protein. This antibody does not cross-react with Bcl-2 or Bcl-XL proteins.
Other Names:
BCL2 associated X protein, apoptosis regulator Bax
Structure:
Forms homodimers and heterodimers with Bcl-2, approximately 21 kD
