Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References:
1. You, WK., et al., 2003. J Biochem (Tokyo) 134:739. 2. Kim, J., et al., 2004. Ann. N. Y. Acad. Sci.1030:95. 3. Ma, D., et al., 2005. Ann. Neurol. 58:182.
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Bax polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.
Description:
Bax is a 21 kD pro-apoptotic protien known to regulate apoptosis. Bax is found in the cytoplasm, mitochondria, and nucleus and is highly expressed in hematopoietic stem cells, the ovary, and in the lymph node. Bax binds the anti-apoptotic protein Bcl-2 as a heterodimer or forms homodimers. The relative levels of pro-apoptotic proteins such as Bax and anti-apoptotic proteins such as Bcl-2 determines whether cell death will occur following an apoptotic stimulus. Bax accelerates the opening of mitochondrial VDAC altering membrane potential and allowing cytochrome c to pass out of the mitochondria into the cytosol to initiate downstream caspase activation. p53 can transcriptionally activate the Bax gene to induce apoptosis. Bax has been shown to be mutated in some human cancers. The Poly6251 antibody has been reported to be useful for Western blotting, immunoprecipitation, and immunohistochemistry of human, mouse, and rat Bax protein.
Other Names:
BCL2 associated X protein, apoptosis regulator Bax
Structure:
Forms homodimers and heterodimers with Bcl-2, approximately 21 kD
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