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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a starting dilution of 1:100. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Zubovych I, et al. 2006. Proc Natl Acad Sci 103:15523. 2. Emerson V, et al. 2009. J. Virol. 1128:1641. PubMed
MCF-7 whole cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-BAP37 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Immunofluorescent microscope analysis of Hela cells using anti-BAP37 polyclonal antibody (red). Nuclei were stain with DAPI (blue).
BAP37, (also known as B-cell receptor-associated protein, repressor of estrogen receptor activity, and prohibitin 2) is a 37 kD erythrocyte band 7 integral membrane protein that contains an SPFH domain. BAP37 is located in the inner mitochondrial membrane that selectively potentiates the inhibitory effectiveness of antiestrogen-occupied ER. BAP37 plays a possible role in cell aging and acts as a mitochondrial protein chaperone. This protein has been shown to interact with the estrogen receptor, prohibitin, and IgM. The Poly6118 antibody has been shown to be useful for Western blotting of the human and mouse BAP37 protein.
Erythrocyte band 7 integral membrane protein, SPFH domain; 37 kD
Distribution:
Inner mitochondrial membrane, integral membrane protein
Function:
Selectively potentiates inhibitory effectiveness of antiestrogen-occupied ER; possible role in cell aging; mitochondrial protein chaperone
Interaction:
Estrogen receptor, prohibitin, IgM
Antigen References:
1. Terashima M, et al. 1994. EMBO J. 13:3782. 2. Montano M, et al. 1999. P. Natl. Acad. Sci. USA 96:6947 3. Nijtmans L, et al. 2000. EMBO J. 19:2444. 4. Piper P, et al. 2002. Aging Cell 1:149.
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Purified anti-BAP37
MCF-7 whole cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-BAP37 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Immunofluorescent microscope analysis of Hela cells using anti-BAP37 polyclonal antibody (red). Nuclei were stain with DAPI (blue).
