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Purified anti-Artemis Antibody
Purified anti-Artemis Antibody
619801 50 µl (5 Western blots) $75.00     
619802 200 µl (20 Western blots) $225.00     
Product Details
Clone: Poly6198
Isotype: Rabbit IgG
Reactivity: Human
Immunogen: Peptide-KLH (KGLRAPTLKRRLE)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by antigen-affinity chromatography.
Storage & Handling: Upon receipt, store frozen at -20° C.
Application: WB, IF
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
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 Immunofluorescent microscope analysis of Hela
Immunofluorescent microscope analysis of Hela cells using anti-Artemis polyclonal antibody (poly6198) (red). α-tubulin (clone 10D8) has been labeled with green and nuclei were stain with DAPI (blue).

MOLT-4 cell nuclear extracts were
MOLT-4 cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Artemis antibody (clone Poly6198). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.


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Description: Artemis is a widely expressed 78 kD protein involved in non-homologous end joining. This protein has been reported to be involved in DNA double-strand break repair and the processing of hairpin coding ends during VDJ recombination. Artemis has endonuclease and exonuclease activity and has been shown to be mutated in human severe combined immune deficiency disorder (T-B- radiosensitive SCID). Artemis is phosphorylated by DNA-PKcs; phosphorylation regulates intrinsic endonuclease and exonuclease activity. Artemis has been shown to form a complex with DNA-PKcs. The Poly6198 antibody has been shown to be useful for Western blotting of the human Artemis protein.
Other Names: Artemis protein
Structure: Protein involved in non-homologous end joining, predicted molecular weight approximately 78 kD
Distribution: Widely expressed
Function: Involved in DNA double-strand break repair and the processing of hairpin coding ends during VDJ recombination. Has endonuclease and exonuclease activity. Mutated in human severe combined immune deficiency disorder (T-B- radiosensitive SCID)
Regulation: Phosphorylation by DNA-PKcs regulates intrinsic endonuclease and exonuclease activity
Modification: Phosphorylation
Interaction: Forms a complex with DNA-PKcs
Antigen
References: 		1. Moshous D, et al. 2001. Cell 105:77
2. Ma Y, et al. 2002. Cell 108:781.
3. Bassing CH, et al. 2002. Cell 109:S45 (review).
4. Rooney S, et al. 2003. J. Exp. Med. 197:553.
GeneID: 430764
Latest Publications: View the latest Artemis articles on HighwirePress.com
UniProt: View information about Artemis on UniProt.org
Keywords: Purified anti-Artemis, Poly6198, Purified, Artemis protein , Human, Immunology, Antibodies
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  • Purified anti-Artemis
    Immunofluorescent microscope analysis of Hela

    Immunofluorescent microscope analysis of Hela cells using anti-Artemis polyclonal antibody (poly6198) (red). α-tubulin (clone 10D8) has been labeled with green and nuclei were stain with DAPI (blue).

    MOLT-4 cell nuclear extracts were

    MOLT-4 cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-Artemis antibody (clone Poly6198). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.

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