Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a dilution range of 1:100~1:400. It is recommended that the reagent be titrated for optimal performance for each application.
MCF7 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Artemis antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Immunofluorescent microscope analysis of Hela cells using anti-Artemis polyclonal antibody (poly6198) (red). α-tubulin (clone 10D8) has been labeled with green and nuclei were stain with DAPI (blue).
Description:
Artemis is a widely expressed 78 kD protein involved in non-homologous end joining. This protein has been reported to be involved in DNA double-strand break repair and the processing of hairpin coding ends during VDJ recombination. Artemis has endonuclease and exonuclease activity and has been shown to be mutated in human severe combined immune deficiency disorder (T-B- radiosensitive SCID). Artemis is phosphorylated by DNA-PKcs; phosphorylation regulates intrinsic endonuclease and exonuclease activity. Artemis has been shown to form a complex with DNA-PKcs. The Poly6198 antibody has been shown to be useful for Western blotting of the human Artemis protein.
Other Names:
Artemis protein
Structure:
Protein involved in non-homologous end joining, predicted molecular weight approximately 78 kD
Distribution:
Widely expressed
Function:
Involved in DNA double-strand break repair and the processing of hairpin coding ends during VDJ recombination. Has endonuclease and exonuclease activity. Mutated in human severe combined immune deficiency disorder (T-B- radiosensitive SCID)
Regulation:
Phosphorylation by DNA-PKcs regulates intrinsic endonuclease and exonuclease activity
Modification:
Phosphorylation
Interaction:
Forms a complex with DNA-PKcs
Antigen References:
1. Moshous, D., et al., 2001. Cell 105:77 2. Ma, Y., et al., 2002. Cell 108:781. 3. Bassing, C.H., et al., 2002. Cell 109:S45 (review). 4. Rooney, S., et al., 2003. J. Exp. Med. 197:553.
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