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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Hela nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-APC8 antibody (clone Poly6114). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
APC8 (anaphase-promoting complex subunit 8) is a member of the E3 enzyme family. This protein contains TPR repeats and has a molecular weight of approximately 68 kD. The APC8 protein is located in the nucleus during interphase and the centrosome during metaphase/anaphase. This protein is required for mitotic anaphase transition. The APC8 protein functions with other members of the APC complex as a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC8 protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1. APC8 is phosphorylated by cdk1-cyclinB and PLK-1. The Poly6114 antibody has been shown to be useful for Western blotting of the human and mouse APC8 protein.
Nuclear during interphase, centrosome during metaphase/anaphase
Function:
Required for mitotic anaphase transition. Multisubunit cell cycle ubiquitin ligase, regulates sister chromatid separation by degrading securins. Involved in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, spindle elongation
Regulation:
Complex inactivated by protein kinase A (PKA) pathway. Activated by CDC20, Cdh1
Modification:
Phosphorylation by cdk1-cyclin B, Plk1
Interaction:
Anaphase promoting complex composed of eight protein subunits APC1-8, APC9-11, Cdc26, Mnd2, and Swm1
Antigen References:
1. Zachariae W, et al. 1999. Genes Dev. 13:2039. 2. Yamashita Y, et al. 1999. Genes Cells. 4:445. 3. Wang Q, et al. 2003. Oncogene. 22:1486. 4. Hall M, et al. 2003. J. Biol. Chem. 278:16698.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-APC8
Hela nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-APC8 antibody (clone Poly6114). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
