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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For immunofluorescence microscopy: Use a starting dilution of 1:100. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The Poly6116 antibody has been shown to be useful for Western blotting of the human and mouse APC11 protein.
MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-APC11 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Overnight nocodazole treated Hela cells stained with purified rabbit polyclonal antibody against APC11 followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI.
APC11 (anaphase-promoting complex subunit 11) is a member of the E3 enzyme family. This protein contains a RING-H2 domain and has a molecular weight of approximately 9.8 kD. The APC11 protein is distributed diffusely in the cytoplasm and is located in the nucleus with discrete accumulation in granular structures. The APC11 protein is a probable catalytic unit in the APC complex. The APC11 protein functions with other members of the APC complex as a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC11 protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1. In addition to the APC complex proteins, APC11 has been shown to interact with Ubc4.
Other Names:
Anaphase-promoting complex subunit 11
Structure:
E3 enzyme family, RING-H2 domain; 9.8 kD
Distribution:
Distributed diffusely in cytoplasm, nucleus with discrete accumulation in granular structures
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-APC11
MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-APC11 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Overnight nocodazole treated Hela cells stained with purified rabbit polyclonal antibody against APC11 followed by Rhodamine Red-X conjugated goat anti-rabbit IgG and DAPI.
