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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Jurkat nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-APC1 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
APC1 (anaphase-promoting complex subunit 1) is a meiotic checkpoint regulator that is a member of the E3 enzyme family. This protein contains Rpn1/2 repeats and has a molecular weight of approximately 216 kD. The APC1 protein is located in the nucleus during interphase, and at the centrosome during metaphase/anaphase. This protein comprises one subunit of the anaphase promoting complex including APC1-8, and other probable complex proteins APC9-11, Cdc26, Mnd2, Swm1. The APC1 protein functions as a probable scaffold for complex assembly, a multisubunit cell cycle ubiquitin ligase, and a regulator of sister chromatid separation by degrading securins. In addition, this protein functions in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, and spindle elongation. The APC complex is inactivated by protein kinase A and is activated by CDC20 and Cdh1. The APC1 protein is phosphorylated by Cdk1-cyclin B and can also be ubiquitinated and targeted for degradation. The Poly6017 antibody has been shown to be useful for Western blotting of the human APC1 protein.
Nuclear during interphase, centrosome during metaphase/anaphase
Function:
Probable scaffold for complex assembly, multisubunit cell cycle ubiquitin ligase. Regulates sister chromatid separation by degrading securins. Involved in ubiquitin-dependent cyclin catabolism, metaphase/anaphase transition, spindle elongation
Regulation:
Complex inactivated by protein kinase A (PKA) pathway. Activated by CDC20, Cdh1
Modification:
Phosphorylation by Cdk1-cyclin B, Ubiquitination
Interaction:
Anaphase promoting complex composed of eight protein subunits APC1-8, APC9-11, Cdc26, Mnd2, and Swm1
Antigen References:
1. Zachariae W, et al. 1999. Genes Dev. 13:2039. 2. Jorgensen P, et al. 2001. Gene 262:51. 3. Golan A, et al. 2002. J. Biol. Chem. 277:15552. 4. Hall M, et al. 2003. J. Biol. Chem. 278:16698.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-APC1
Jurkat nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-APC1 antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
