Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 15 µg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Formalin-fixed paraffin-embedded human kidney tissue was stained with 20G10 at 15 µg/ml and developed with an alkaline phosphatase chromogen substrate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
Jurkat cell extract (Lane 1) or NIH3T3 cell extract (Lane 2) was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-AND-1 (Clone 20G10) antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
Description:
The 20G10 monoclonal antibody recognizes both human and mouse AND-1 (WDHD1) also known as WD repeat and HMG box DNA binding protein 1, acidic nucleoplasmic DNA-binding protein 1. AND-1 (WDHD1) is a nuclear and nucleolar protein that contains multiple WD40 repeats, and one HMG region. AND-1 (WDHD1) has a predicted molecular weight of 126 kD and can form homodimers. WDHD1 is expressed in the brain, epidermis, liver, and stomach. During mitosis, AND-1 (WDHD1) is transiently expressed in nucleoplasm. AND-1 (WDHD1) can bind to DNA with high affinity and may be involved in protein-protein interactions. The role of AND-1 (WDHD1) is thought to be in chromatin assembly, transcription and DNA replication. AND-1 (WDHD1) has been reported to be phosphorylated on S1041. The 20G10 antibody has been shown to be useful for Western blotting.
Other Names:
WD repeat and HMG box DNA binding protein 1, acidic nucleoplasmic DNA-binding protein 1
Structure:
Contains multiple WD40 repeats, one HMG region. Predicted molecular weight 126 kD. Forms homodimers.
Distribution:
Found in the nucleus and nucleolus. Expressed in brain, epidermis, liver, and stomach. During mitosis transiently expressed in nucleoplasm.
Function:
Binds DNA with high affinity; may be involved in protein-protein interactions. Thought to function in chromatin assembly, transcription and DNA replication.
Modification:
Phosphorylation (S1041)
Antigen References:
1. Kohler, A., et al. 1997. J. Cell Sci. 110:1051.
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