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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-α-adducin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
The α-adducin protein is a ubiquitously expressed cytoskeletal protein that binds with high affinity to Ca2+/calmodulin and acts as a substrate for protein kinases A and C. α-addducin is a 80-120 kD member of the aldolase class II family and adducin subfamily. This protein has three isoforms that interact as heterodimers α/β, and α/γ. The molecular weights for the various isoforms are α=120 kD; β=110kD; γ=80kD. This protein has been reported to promote the assembly of the spectrin-actin network. Polymorphisms in α-adducin are associated with cardiovascular disease and hypertension. α-adducin has been reported to interact with calmodulin. When phosphorylated by Rho-kinase, α-adducin can regulate membrane ruffling and cell motility. The Poly6170 antibody has been shown to be useful for Western blotting of mouse and human α-adducin protein.
Other Names:
ADD-1
Structure:
Aldolase class II family, adducin subfamily, heterodimerizes α/β, α/γ. Multiple isoforms α-120 kD, β-110 kD, γ-80kD
Distribution:
Ubiquitously expressed cytoskeletal protein
Function:
Binds with high affinity to Ca2+/calmodulin, substrate for protein kinases A and C, promotes the assembly of spectrin-actin network. Polymorphisms associated with cardiovascular disease and hypertension
Regulation:
Phosphorylation by Rho-kinase regulates membrane ruffling, cell motility
Modification:
Phosphorylation
Interaction:
Calmodulin
Antigen References:
1. Fukata Y, et al. 1999. J. Cell Biol. 145:347. 2. Joshi R, et al. 1991. J. Cell. Biol. 115:665. 3. Lin B, et al. 1995. Genomics 25:93.
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Purified anti-α-Adducin
HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-α-adducin antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
