Antibodies
Login
Search   
Purified anti-AIP1 Antibody
Purified anti-AIP1 Antibody
645601 25 µg €84     
Product Details
Clone: 13F10
Isotype: Rat IgG
Reactivity: Mouse
Immunogen: Full length recombinant protein expressed in E Coli
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4 °C.
Application:

WB - Quality tested
Recommended Usage:

Each lot of this antibody has been quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5 - 1 µg per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Enter Lot#:   
Application
References:

1. Li J, et al. 2007. Nature Cell Biology 9:276
NIH/3T3 (left) and Jurkat (right)
NIH/3T3 (left) and Jurkat (right) cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with purified anti-mAIP1 (Clone 13F10) antibody. Proteins were visualized using an HRP goad anti-rat secondary antibody conjugate and a chemiluminescent detection system.


Compare all formats



Description:

AIP1(actin-interaction protein 1) is a 67 kD WD repeat protein known to regulate the depolymerization of actin filaments by cofilin and is conserved in organisms ranging from yeast to mammals. Recently, it was reported that caspase-11 and AIP1 cooperatively promote cofilin-mediated actin depolymerization.
Other Names: Actin-interacting protein 1
Structure: A protein of 67-kd WD-repeat protein. It contains as many as 10 WD repeats which is characterized by homologous sequence of around 40 amino acids with Trip-Asp(WD) at the carboxyl ends.
Function: Enhance actin filament disassembly in the presence of actin-depolymerizing factor cofilin.
Ligand Receptor: Caspase-11 interacts physically with AIP1 to promote cofilin-mediated actin depolymerization.
Interaction: AIP1 binds cofilin/actin depolymerization factor.
Antigen
References:

1. Voegtli WC, et al. 2003 J. Biol. Chem. 278:34373
2. Mohri K, et al. 2004. J. Biol. Chem. 279:31697
GeneID: 22388
Latest Publications: View the latest AIP1 articles on HighwirePress.com
UniProt: View information about AIP1 on UniProt.org
Keywords: Purified anti-AIP1, 13F10, Purified, Actin-interacting protein 1, Mouse, Immunology, Antibodies
Related Products
DescriptionCloneApplications
Purified Goat anti-rat IgG (minimal x-reactivity)Poly4054FC, ELISA, WB
Cell Staining BufferFC, ICC, ICFC
RBC Lysis Buffer (10X)FC, ICFC
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
x
Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-AIP1
    NIH/3T3 (left) and Jurkat (right)

    NIH/3T3 (left) and Jurkat (right) cell lysates were resolved by electrophoresis, transferred to nitrocellulose and probed with purified anti-mAIP1 (Clone 13F10) antibody. Proteins were visualized using an HRP goad anti-rat secondary antibody conjugate and a chemiluminescent detection system.

x
Copyright © 2011 BioLegend, Inc. All rights reserved.