Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application
Application References:
1. Weaver, B.K. et al. 2007. Mol. Cell. Biol. 27:4603.
Western blot analysis of extracts from untreated mouse bone marrow-derived macrophages (BMM) (lane 1) or 100 ng/ml LPS plus 20 ng/ml mIL-10 treated BMM (lane 2), using anti-ABIN-3, clone Tgr21A5.
Description:
ABIN-3, also known as LIND, was originally identified as a gene induced in monocytes upon infection by Listeria monocytogenes. ABIN-3 is induced upon stimulation by IL-10.Expression of human ABIN-3 in cells inhibited NF-kappa-B activation and proinflammatory gene expression in response to TNF, IL-1, and TLR ligands.In mice, ABIN-3 lacks the AHD2 domain, which explains its inability to inhibit NF-kappa-B in mice.
Other Names:
TNFAIP3-interacting protein 3, TNIP3, LIND, Listeria-induced gene protein, A20-binding inhibitor of NK-kappa-B activation 3 < ABIN3, LIND>
Structure:
ABIN family of proteins. Predicted MW, 24 kD
Distribution:
Expressed in macrophages in response to LPS and IL-10
Function:
ABIN-3 expression suppresses NF-kappa-B activation and induction of proinflammatory genes in humans but not mice
Regulation:
Upregulated by LPS plus IL-10
Antigen References:
1. Staege, H. et al. 2001. Immunogenetics 53:105. 2. Wullaert, A. et al. 2007. J. Biol. Chem. 282:81
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