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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application References:
1. Nakav, S., et al. 2011. Am J Respir Cell Mol Biol. PubMed.
Western blot analysis of extracts from Hela cells, using anti-14-3-3 ζ, Poly6378.
14-3-3 zeta is a 28 kD protein, also known tyrosine 3-monooxygenase/tryptophan 5-monoxygenase activation protein, zeta polypeptide that belongs to the family of 14-3-3 proteins.This family of proteins mediates signal transduction and cell cycle progression by binding to phosphoserine and phosphothreonine motifs on a large number of target molecules. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C.There are eight known isoforms of 14-3-3: alpha, beta, gamma, delta, epsilon, zeta, eta, and theta.The Poly6378 antibody has been shown to be useful for western blotting of human 14-3-3 zeta protein.
Other Names:
Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ
Structure:
14-3-3 protein superfamily, homodimer, 28 kD
Distribution:
Cytoplasm, Melanosome.
Function:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif.
Purified anti-14-3-3 ζ, Poly6378, Purified, Protein kinase C inhibitor protein 1, KCIP-1, YWHAZ , Human, Cross-Reactivity: Mouse (very weak), Immunology, Antibodies
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-14-3-3 ζ
Western blot analysis of extracts from Hela cells, using anti-14-3-3 ζ, Poly6378.
