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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
COA:
Application Notes:
The Poly6379 antibody has been shown to be useful for western blotting of human 14-3-3 epsilon protein.
Hela cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-14-3-3 ε antibody (clone Poly6379). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
14-3-3ε is a 29 kD protein that belongs to the family of 14-3-3 proteins. This family of proteins mediates signal transduction and cell cycle progression by binding to phosphoserine and phosphothreonine motifs on a large number of target molecules. 14-3-3 proteins activate tyrosine and tryptophan hydroxylases as well as protein kinase C. There are eight known isoforms of 14-3-3: alpha, beta, gamma, delta, epsilon, zeta, eta, and theta.
Other Names:
14-3-3E, YWHAE
Structure:
14-3-3 protein superfamily, homodimer, 29 kD
Distribution:
Cytoplasm
Function:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif.
1. Jin DY, et al. 1996. Nature 382:308. 2. Conklin DS, et al. 1995. Proc. Natl. Acad. Sci. USA 92:7892. 3. Zannis-Hadjopoulos M, et al. 2008. Trends Biochem. Sci. 33:44.
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This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-14-3-3 ε
Hela cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-14-3-3 ε antibody (clone Poly6379). Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection.
