Full length human 14-3-3 epsilon recombinant protein
Formulation:
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage & Handling:
Upon receipt, store frozen at -20°C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Description:
14-3-3epsilon is a 29 kD protein that belongs to the family of 14-3-3 proteins.This family of proteins mediates signal transduction and cell cycle progression by binding to phosphoserine and phosphothreonine motifs on a large number of target molecules.14-3-3 proteins activate tyrosine and tryptophan hydroxylases and protein kinase C.There are eight known isoforms of 14-3-3: alpha, beta, gamma, delta, epsilon, zeta, eta, and theta.The Poly6379 antibody has been shown to be useful for western blotting of human 14-3-3 epsilon protein.
Other Names:
14-3-3E, YWHAE < YWHAE>
Structure:
14-3-3 protein superfamily, homodimer, 29 kD
Distribution:
Cytoplasm
Function:
Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif.
1. Jin D.-Y., et al. 1996. Nature 382:308. 2. Conklin D.S., et al. 1995. Proc. Natl. Acad. Sci. U.S.A. 92:7892. 3. Zannis-Hadjopoulos M., et al. 2008. Trends Biochem. Sci. 33:44.
