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The suggested use of this solution is 10 µl per 106 cells in 0.5 ml/test, and incubate for 15 minutes at 4 °C before analysis. Caution: This solution is toxigenic and mutagenic; handle with care.
COA:
Application Notes:
Propidium Iodide Solution can be used in evaluation of apoptosis, cell viability and cell cycle analysis by flow cytometry.
Application References:
1. Vermes I, et al.1995. J. Immunol. Methods 184:39. 2. Darzynkiewicz Z, et al. 1992. Cytometry 13(8):795. 3. Douglas RS, et al. 1995. J. Immunol. Methods 188:219. 4. Sakimoto I, et al. 2006. Cancer Research 66:2287.PubMed
Description:
Propidium iodide (PI) is a fluorescent dye that binds to DNA. When excited by 488nm laser light, it can be detected with 562-588nm band pass filter. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.
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