Propidium Iodide Solution
Propidium Iodide Solution
421301 2 ml $45.00 Qty:

Product Details

Formulation: Phosphate-buffered saline, pH 7.2, containing 0.09% sodium azide.
Concentration: 0.5 mg/ml
Storage & Handling: The solution should be stored undiluted between 2°C and 8°C, and protect from light. Caution: This solution contains hazardous material; handle with care.

FC - Quality tested

Recommended Usage:

The suggested use of this solution is 10 µl per 106 cells in 0.5 ml/test, and incubate for 15 minutes at 4 °C before analysis. Caution: This solution is toxigenic and mutagenic; handle with care.

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
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Propidium Iodide Solution can be used in evaluation of apoptosis, cell viability and cell cycle analysis by flow cytometry.


  Publication Library

See Propidium Iodide spectral data

Cell Surface Immunofluorescence Staining Protocol
Propidium Iodide Cell Cycle Staining Protocol


Propidium iodide (PI) is a fluorescent dye that binds to DNA. When excited by 488nm laser light, it can be detected with 562-588nm band pass filter. It is commonly used in evaluation of cell viability or DNA content in cell cycle analysis by flow cytometry.

  Learn more about Live Cell/Dead Cell Discrimination.

Other Names: PI
GeneID: NA
Latest Publications: View the latest Propidium Iodide Solution articles on
Keywords: Propidium Iodide Solution, Solution, PI, Flow Cytometry, Immunology, Antibodies

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Product FAQs

Why washing is not recommended post 7AAD or PI addition for viability check?
These dyes bind in equilibrium with DNA. Therefore external dye concentration must be maintained during analysis and the dye should not be washed out.
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Buffers, Solutions & Chemicals
Buffers, Solutions & Chemicals > FC/ICFC Buffers & Solutions
Buffers, Solutions & Chemicals > Live/Dead
Cell Biology > Cell Cycle/DNA Replication > Propidium Iodide Solution

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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