BioLegend Product Reviews
Product: PE anti-mouse/human CD44
Catalog No.: 103008
Jacqueline Kimmey, Graduate Student Washington University

Detection of CD44 in Mice (PE)
Overall: Product Quality: Ease of Use:
Reliable flow cytometry reagent.
Experimental Design

Application: Flow cytometry
Cells used: Murine lung or lymph node
Brief Protocol: Collect cells, wash and stain in FACS buffer. Block with FcBlock. Stain at 1:200 4oC for 20 minutes. Detect by flow cytometry. Fixable in PFA if desired.
Results Summary: Consistent staining, populations are more of a smear that a distinct population but that is likely due to the antigen not the antibody or product formulation and makes sense based on what I would predict for CD44 expression.
Additional Notes: Will use again. No issues noted.
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Publication Status

This data has not been published.
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