BioLegend Product Reviews
Product: Brilliant Violet 605™ anti-mouse Ki-67
Catalog No.: 652413
Jacqueline Kimmey, Graduate Student Washington University in St. Louis

Great Ab and Color (Brilliant Violet 605™ anti-mouse Ki-67)
Overall: Product Quality: Ease of Use:
Ab works as expected, bright and clear staining.
Experimental Design

Application: Flow Cytometry
Cells used: Murine bone marrow and murine blood cells
Brief Protocol: Prep cells for flow, as normal. Perform surface staining and then fix in PFA.
Perm with BioLegend FoxP3 / Fix Perm, as per protocol, (this worked better for me than the TrueNuclear™ buffer).
Stain cells with anti-Ki67 (1:200 for 30 min, room temp).
Wash in FACS buffer, analyze cells.
Results Summary: Clear positive and negative populations.
Additional Notes:
Data Image

Click on the image for full size data.
Publication Status

This data has not been published.
See all Reviews
Related Pages:

Reviews Library
Brilliant Violet™
Brilliant Violet™ Mobile Application
Reviews Library
Multicolor Panel Selector
Fluorescence Spectra Analyzer
Fluorophore Brightness Index
Custom Panel Construction
Fluorophore Equivalency Tool
Flow Cytometry Mobile Application
Fluorescence Spectra Analyzer Mobile Application
Forgot your password? Reset Password
Request an Account