BioLegend Product Reviews
Product: ELISA MAX™ Deluxe Set Mouse IL-1β
Catalog No.: 432606
Rissiek Björn, Dr. University Medical Center Hamburg-Eppendorf

Mouse IL-1β ELISA MAX™ Deluxe for the Detection IL-1β Released from Microglia In Vitro
Overall: Product Quality: Ease of Use:
We continuously use this ELISA kit for the detection of IL-1β in the supernatant of mixed glial cell cultures.
Experimental Design

Application: Detection of IL-1β release from microglia
Cells used: Microglia
Brief Protocol: We generated mixed glial cell cultures from neonatal mice (p0-2) in 24-well plates and stimulated the cells for 2h with LPS to induce production of pro-IL-1β in microglia. We subsequently stimulated cells with 1.5mM ATP to induce P2X7-mediated inflammasome formation and cleavage of pro-IL-1β and release of IL-1β into the supernatant. Latter was quantified using Mouse IL-1β ELISA MAX™ Deluxe.
Results Summary: Supernatants contained 100-300pg/ml mouse IL-1β, depending on the number of microglia in the mixed glial cell culture.
Additional Notes:
Data Image

Click on the image for full size data.
Publication Status

This data has not been published.
See all Reviews
Related Pages:

Reviews Library
ELISA Kits and Sets
ELISA Mobile Application
Reviews Library
Multicolor Panel Selector
Fluorescence Spectra Analyzer
Fluorophore Brightness Index
Custom Panel Construction
Fluorophore Equivalency Tool
Flow Cytometry Mobile Application
Fluorescence Spectra Analyzer Mobile Application
Login/Register
Forgot your password? Reset Password
Request an Account