BioLegend Product Reviews
Product: ELISA MAX™ Deluxe Set Human IL-15
Catalog No.: 435104
Sojan Abraham, Post Doc TTUHSC

Good ELISA Kit for Detecting Human IL-15
Overall: Product Quality: Ease of Use:
Was able to quantify IL-15 in cell culture supernatants using this ELISA kit.
Experimental Design

Application: ELISA
Cells used: Cell culture supernatants
Brief Protocol: 1. Plates were coated with capture antibody overnight at 4 degree.
2. Plates were washed three times and blocked for 1 hr with 1X assay diluent.
3. Plates were washed three times and incubated with IL-15 standards and unknown samples for two hours.
4. Samples were washed three times and incubated with detection antibody for 1 hr.
5. Plates were washed three times and incubated with Avidin HRP for 30 min.
6. Plates were washed 4 times and incubated with 100 ul substrate solution for 15 minutes.
7. After 15 minutes 100 ul stop solution was added and absorbance was measured at 450.
Results Summary: Using this ELISA kit I was able to quantify the amount of IL-15 in cell culture supernatants.
Additional Notes:
Data Image

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Publication Status

This data has not been published.
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