BioLegend Product Reviews
Product: ELISA MAX™ Deluxe Set Human IL-17A
Catalog No.: 433914
Sojan Abraham, Post Doc TTUHSC

Very Good ELISA Kit for Detecting Human IL-17A
Overall: Product Quality: Ease of Use:
Was able to detect human IL- 17A from cell culture supernatants.
Experimental Design

Application: ELISA
Cells used: Cell culture supernatants
Brief Protocol: 1. Plates were coated with capture antibody overnight at 4 degree.
2. Plates were washed three times and blocked for 1 hr with 1X assay diluent.
3. Plates were washed three times and incubated with IL-17A standards and unknown samples for two hours.
4. Samples were washed three times and incubated with detection antibody for 1 hr.
5. Plates were washed three times and incubated with Avidin HRP for 30 min
6. Plates were washed 4 times and incubated with 100 ul substrate solution for 15 minutes.
7. After 15 minutes 100 ul stop solution was added and absorbance was measured at 450.
Results Summary: Was able to detect IL-17A from cell culture supernatants.
Additional Notes:
Data Image

Publication Status

This data has been published.

Pubmed
See all Reviews
Related Pages:

Reviews Library
ELISA Kits and Sets
ELISA Mobile Application
Reviews Library
Multicolor Panel Selector
Fluorescence Spectra Analyzer
Fluorophore Brightness Index
Custom Panel Construction
Fluorophore Equivalency Tool
Flow Cytometry Mobile Application
Fluorescence Spectra Analyzer Mobile Application
Login/Register
Forgot your password? Reset Password
Request an Account