BioLegend Product Reviews
Product: Human TruStain FcX™ (Fc Receptor Blocking Solution)
Catalog No.: 422302
Jonathan Eby, Graduate Student Loyola University Chicago

Fc Block and THP-1 Cells (Human TruStain FcX™)
Overall: Product Quality: Ease of Use:
Increased antibody sensitivity and reduced background after Fc Blocking.
Experimental Design

Application: Flow cytometry
Cells used: THP-1 cells
Brief Protocol: Cells spun down directly from incubator. Cells were incubated 10 minutes in PBS + 2% human serum in addition to the Human TruStain FcX block. Following incubation, primary antibodies were added and incubated at 4 C for 30 minutes, washed 2x, then incubated with secondary antibodies for 30 minutes at 4 C. Cells were washed 2x, then immediately ran on a cytometer.
Results Summary: Human TruStain FcX greatly reduced background staining seen typically from incubation with secondary antibody on this cell line.
Additional Notes:
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Publication Status

This data has not been published.
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Breaking Buffers
Flow Cytometry Buffers
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