Catalog # / Size:
620401 / 50 µl (5 Western blots)
620402 / 200 µl (20 Western blots)
620402 / 200 µl (20 Western blots)
HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal antibody against ch-TOG. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
Clone:
Poly6204
Isotype:
Rabbit IgG
Reactivity:
Human
Immunogen:
Partial recombinant protein, containing approximately 200 amino acids from the C-terminus
Formulation:
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation:
The antibody was purified by antigen-affinity chromatography.
Storage:
Upon receipt, store frozen at -20° C.
Application:
WB
Recommended Usage:
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 μl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application
Notes:
Notes:
This antibody is not suitable for immunoprecipitation.
Application
References:
References:
1. Appierto, V., et al. 2009. Mol Cancer Res. 8:3360. PubMed.
Description:
ch-TOG (also known as colonic and hepatic tumor over-expressed protein) is a 218 kD member of the TOG/XMAP215 family containing heat repeats. This protein is localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis. Ch-TOG is believed to function in spindle microtubule organization and has been shown to interact with TACC1, TACC3,and tubulin. The Poly6204 antibody has be shown to be useful for Western blotting of the human ch-TOG protein.
Other Names:
Colonic and hepatic tumor over-expressed protein, KIAA0097
Structure:
TOG/XMAP215 family, heat repeats; 218 kD
Distribution:
Interphase, perinuclear cytoplasm; mitosis, spindle poles, centrosomes
Function:
Spindle microtubule organization
Interaction:
TACC1, TACC3, tubulin
Antigen
References:
References:
1. Charrasse, S., et al., 1995. Eur. J. Biochem. 234:406.
2. Garcia, M., et al., 2001. EMBO J. 20:3389.
3. Lauffart, B., et al., 2002. Biochem. J. 363:195.
4. Gergely, F., et al., 2003. Genes Dev. 17:336.
2. Garcia, M., et al., 2001. EMBO J. 20:3389.
3. Lauffart, B., et al., 2002. Biochem. J. 363:195.
4. Gergely, F., et al., 2003. Genes Dev. 17:336.
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