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The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
This reagent is developed for immunofluorescent staining for flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. It is highly recommended that the reagent be titrated for optimal performance for each application.
* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.
COA:
Application Notes:
The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofleorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 400622) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400644) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application References:
1. Cervantes-Barragan L, et al. 2007. Blood 109:1131. 2. Zeiser R, et al. 2007. Blood 109:2225. 3. Sasaki K, et al. 2008. J. Immunol. 181:104. PubMed 4. Duan J, et al. 2008. P. Natl. Acad. Sci. USA 105:5183. PubMed 5. Yi H, et al. 2009. Blood 113:5819. PubMed 6. Schafeer JS, et al. 2010. J. Leukocyte Biol. 87:301. PubMed
The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
PerCP Rat IgG2b, κ Isotype Ctrl, RTK4530, PerCP, Immunology, Antibodies
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
