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PerCP Mouse IgG2b, κ Isotype Ctrl Antibody
PerCP Mouse IgG2b, κ Isotype Ctrl Antibody
400335 25 tests $75.00     
400336 100 tests $180.00     

Product Details

Clone: MPC-11
Isotype: Mouse IgG2b, κ
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions. The solution is free of unconjugated PerCP and unconjugated antibody.
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC, ICFC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. For immunofluorescent staining, the suggested use of this reagent is 5 µl per 106 cells or 5 µl per 100 µl of whole blood.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

COA:
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Application
Notes:

The MPC-11 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofleorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 400324) as negative control.

Application
References:

1. Smed-Sörensen A, et al. 2008. Blood 111:5037. (FA) PubMed
2. Podolin PL, et al. 2008. J. Immunol. 180:7989. (FC) PubMed

Human peripheral blood lymphocytes stained
Human peripheral blood lymphocytes stained with MPC-11 PerCP


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Description:

The MPC-11 immunoglobulin has unknown specificity. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

GeneID: NA
Latest Publications: View the latest IgG2b, kappa Isotype Ctrl articles on HighwirePress.com
UniProt: View information about IgG2b, kappa Isotype Ctrl on UniProt.org
Keywords: PerCP Mouse IgG2b, κ Isotype Ctrl, MPC-11, PerCP, Immunology, Antibodies
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • PerCP Mouse IgG2b, κ Isotype Ctrl
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with MPC-11 PerCP

  • PerCP/Cy5.5 Mouse IgG2b, κ Isotype Ctrl
    Human peripheral blood lymphocytes stained

    Human peripheral blood lymphocytes stained with MPC-11 PerCP/Cy5.5

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