The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC, ICFC
Recommended Usage:
This reagent is developed for immunofluorescent staining for flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. It is highly recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximum emission of 690 nm.
Application Notes:
The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofleorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 400622) as negative control.
Application References:
1. Cervantes-Barragan, L., et al., 2007. Blood 109:1131. 2. Zeiser, R., et al., 2007. Blood 109:2225.
Description:
The isotype of RTK4530 immunoglobulin is rat IgG2b,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
