Antibodies
|
Search   
PerCP/Cy5.5 Rat IgG2b, κ Isotype Ctrl Antibody
PerCP/Cy5.5 Rat IgG2b, κ Isotype Ctrl Antibody
400631 25 µg $80.00 Qty:
    
400632 100 µg $160.00 Qty:
    

Product Details

 
Clone: RTK4530 (See other available formats)
Isotype: Rat IgG2b, κ
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated immunoglobulin.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC, ICFC - Quality tested

Recommended Usage: This reagent is developed for immunofluorescent staining for flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. It is highly recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Cy® and CyDye® are registered trademarks of GE Healthcare.

Excitation
Laser:
Blue Laser (488 nm)
COA:
Enter Lot#:   
Application
Notes:

The RTK4530 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 400622) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400644) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application
References:

  Publication Library

See PerCP/Cy5.5 spectral data







Description:

The isotype of RTK4530 immunoglobulin is rat IgG2b, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

GeneID: NA
Latest Publications: View the latest IgG2b, kappa Isotype Ctrl articles on HighwirePress.com
UniProt: View information about IgG2b, kappa Isotype Ctrl on UniProt.org
Keywords: PerCP/Cy5.5 Rat IgG2b, κ Isotype Ctrl, RTK4530, PerCP/Cy5.5, Flow Cytometry, Intracellular Staining for Flow Cytometry, Immunology, Antibodies


Product Reviews

  Title Rating Date
- There are currently no reviews for this product. Review this product at Biocompare.com to receive a gift card. -
      See more reviews using the Reviews Library


Product FAQs

 
How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?
PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
For a complete list of FAQs on all our products, visit our FAQ page.
 
Related Products

DescriptionCloneApplications


Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
Alexa Fluor® 647 Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
Alexa Fluor® 700 Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
APC Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
APC/Cy7 Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
Biotin Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
Brilliant Violet 421™ Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
Brilliant Violet 510™ Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
Brilliant Violet 570™ Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
Brilliant Violet 605™ Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
Brilliant Violet 650™ Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
Brilliant Violet 711™ Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
Brilliant Violet 785™ Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
FITC Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
LEAF™ Purified Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC, WB, IP, ICC, IF, IHC, FA
Pacific Blue™ Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
PE Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
PE/Cy5 Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
PE/Cy7 Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
PerCP Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC
Purified Rat IgG2b, κ Isotype CtrlRTK4530FC, ICC, ICFC, IF, IHC, IP, WB
Ultra-LEAF™ Purified Rat IgG2b, κ Isotype CtrlRTK4530FC, ICFC, WB, IP, ICC, IF, IHC, FA


Related Pathways

Pathway


Related Categories

Category
Isotype Controls > Rat IgG2b, κ

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
x
Copyright © 2013 BioLegend, Inc. All rights reserved.