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PerCP/Cy5.5 Rat IgG2a, κ Isotype Ctrl Antibody
Cat. # Size Price  

400531 25 µg $80.00
    
400532 100 µg $205.00
    
Product Details

Clone: RTK2758 (See other available formats)
Isotype: Rat IgG2a, κ
Reactivity: KLH
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated immunoglobulin.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC, ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.Cy® and CyDye® are registered trademarks of GE Healthcare.
Excitation
Laser:
Blue Laser (488 nm)
Application
Notes:
The RTK2758 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 400516) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400544) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Application
References:
  Publication Library

See PerCP/Cy5.5 spectral data





 


Antigen Details

Description: The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.
GeneID: NA
UniProt: View information about Rat IgG2a, kappa Isotype Ctrl on UniProt.org
Keywords: PerCP/Cy5.5 Rat IgG2a, κ Isotype Ctrl, RTK2758, PerCP/Cy5.5, KLH, Flow Cytometry, Intracellular Staining for Flow Cytometry, Immunology, Antibodies
Technical Data Sheet (pdf)
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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