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PerCP/Cy5.5 Rat IgG2a, κ Isotype Ctrl Antibody
PerCP/Cy5.5 Rat IgG2a, κ Isotype Ctrl Antibody
400531 25 µg $80.00 Qty:
    
400532 100 µg $205.00 Qty:
    

Product Details

 
Clone: RTK2758 (See other available formats)
Isotype: Rat IgG2a, κ
Reactivity: KLH
Immunogen: Trinitrophenol + KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated immunoglobulin.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC, ICFC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Cy® and CyDye® are registered trademarks of GE Healthcare.

Excitation
Laser:
Blue Laser (488 nm)
COA:
Enter Lot#:   
Application
Notes:

The RTK2758 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, immunofluorescence microscopy, immunocytochemistry and immunofluorescent staining (surface or intracellular) for flow cytometric analysis. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 400516) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400544) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application
References:

  Publication Library

See PerCP/Cy5.5 spectral data







Description:

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

GeneID: NA
Latest Publications: View the latest Rat IgG2a, kappa Isotype Ctrl articles on HighwirePress.com
UniProt: View information about Rat IgG2a, kappa Isotype Ctrl on UniProt.org
Keywords: PerCP/Cy5.5 Rat IgG2a, κ Isotype Ctrl, RTK2758, PerCP/Cy5.5, KLH, Flow Cytometry, Intracellular Staining for Flow Cytometry, Immunology, Antibodies


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Product FAQs

 
How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?
PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
For a complete list of FAQs on all our products, visit our FAQ page.
 
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DescriptionCloneApplications


Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
Alexa Fluor® 594 Rat IgG2a, κ Isotype CtrlRTK2758IF
Alexa Fluor® 647 Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
Alexa Fluor® 700 Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
APC Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
APC/Cy7 Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
Biotin Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
Brilliant Violet 421™ Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
Brilliant Violet 510™ Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
Brilliant Violet 570™ Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
Brilliant Violet 605™ Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
Brilliant Violet 650™ Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
Brilliant Violet 711™ Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
Brilliant Violet 785™ Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
FITC Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
LEAF™ Purified Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC, WB, IP, ICC, IF, IHC, FA
Pacific Blue™ Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
PE Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
PE/Cy5 Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
PE/Cy7 Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
PE/Dazzle™ 594 Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
PerCP Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC
Purified Rat IgG2a, κ Isotype CtrlRTK2758FC, ICC, IF, IHC, IP, WB
Ultra-LEAF™ Purified Rat IgG2a, κ Isotype CtrlRTK2758FC, ICFC, WB, IP, ICC, IF, IHC, FA


Related Pathways

Pathway


Related Categories

Category
Isotype Controls > Rat IgG2a, κ

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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