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PerCP/Cy5.5 Mouse IgG2a, κ Isotype Ctrl Antibody
PerCP/Cy5.5 Mouse IgG2a, κ Isotype Ctrl Antibody
400251 25 tests $80.00 Qty:
    
400257 25 µg $80.00 Qty:
    
400252 100 tests $205.00 Qty:
    
400258 100 µg $205.00 Qty:
    

Product Details

 
Clone: MOPC-173 (See other available formats)
Isotype: Mouse IgG2a, κ
Formulation: µg format: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Test format: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 0.2% (w/v) BSA (USA origin).
Preparation: The immunoglobulin was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated immunoglobulin.
Concentration: test sizes: lot-specific; µg size: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC, ICFC - Quality tested

Recommended Usage:

Each lot of this mouse IgG2a, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Cy® and CyDye® are registered trademarks of GE Healthcare.

Excitation
Laser:
Blue Laser (488 nm)
COA:
Enter Lot#:   
Application
Notes:

The MOPC-173 immunoglobulin is useful as an isotype-matched control (for the relevant formats) for Western blotting, immunoprecipitation, immunohistochemistry, functional assay, and immunofluorescence microscopy. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 400224) as negative control. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 400264) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application
References:

  Publication Library

See PerCP/Cy5.5 spectral data







Description:

The MOPC-173 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

GeneID: NA
Latest Publications: View the latest IgG2a, kappa Isotype Ctrl articles on HighwirePress.com
UniProt: View information about IgG2a, kappa Isotype Ctrl on UniProt.org
Keywords: PerCP/Cy5.5 Mouse IgG2a, κ Isotype Ctrl, MOPC-173, PerCP/Cy5.5, Flow Cytometry, Intracellular Staining for Flow Cytometry, Immunology, Antibodies


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Product FAQs

 
How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?
PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
IgG2a gene is deleted in some mouse strains, which ones are they?
IgG2a gene is deleted in C57Bl/6, C57Bl/ 10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
For a complete list of FAQs on all our products, visit our FAQ page.
 
Related Products

DescriptionCloneApplications
Cell Staining BufferFC, ICC, ICFC


Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
Alexa Fluor® 488 Mouse IgG2a, κ Isotype Ctrl (ICFC)MOPC-173ICFC
Alexa Fluor® 647 Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
Alexa Fluor® 647 Mouse IgG2a, κ Isotype Ctrl (ICFC)MOPC-173ICFC
Alexa Fluor® 700 Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
APC Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
APC Mouse IgG2a, κ Isotype Ctrl (ICFC)MOPC-173ICFC
APC Mouse IgG2a, κ Isotype Ctrl (FC)MOPC-173FC
APC/Cy7 Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
Biotin Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
Brilliant Violet 421™ Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
Brilliant Violet 510™ Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
Brilliant Violet 570™ Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
Brilliant Violet 605™ Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
Brilliant Violet 650™ Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
Brilliant Violet 711™ Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
Brilliant Violet 785™ Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
FITC Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
FITC Mouse IgG2a, κ Isotype Ctrl (ICFC)MOPC-173ICFC
FITC Mouse IgG2a, κ Isotype Ctrl (FC)MOPC-173FC
LEAF™ Purified Mouse IgG2a, κ Isotype CtrlMOPC-173FC, WB, IP, ICFC, ICC, IF, IHC, FA
Pacific Blue™ Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
PE Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
PE Mouse IgG2a, κ Isotype Ctrl (ICFC)MOPC-173ICFC
PE Mouse IgG2a, κ Isotype Ctrl (FC)MOPC-173FC
PE/Cy5 Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
PE/Cy7 Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
PE/Dazzle™ 594 Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
PerCP Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICFC
Purified Mouse IgG2a, κ Isotype CtrlMOPC-173FC, ICC, IF, IHC, IP, WB
Ultra-LEAF™ Purified Mouse IgG2a, κ Isotype CtrlMOPC-173FC, WB, IP, ICFC, ICC, IF, IHC, FA


Related Pathways

Pathway


Related Categories

Category
Isotype Controls > Mouse IgG2a, κ > MOPC-173
Isotype Controls > Mouse IgG2a, κ

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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