Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
COA:
Application Notes:
While IA8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Additional reported applications (for the relevant formats) include: depletion.
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC) 2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC) 3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC) 4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed 5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed 6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed 7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed 8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed.
C57BL/6 bone marrow cells stained with 1A8 PerCP/Cy5.5
Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in the bone marrow and granulocytes in the periphery.
Other Names:
Ly6G
Structure:
A 21-35 kD GPI-anchorded membrane protein
Distribution:
Expressed on majority of myeloid cells in the bone marrow and granulocytes in the periphery. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 purified, followed by anti-rat IgG PE (myeloid cells were gated for analysis)
Biotin anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 biotin, followed by Sav-PE (gated on myeloid cells)
FITC anti-mouse Ly-6G
BALB/cmouse bone marrow cells stained with 1A8 FITC (gated on myeloid cells)
PE anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 PE (gated on myeloid cells)
Alexa Fluor® 647 anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells stained with 1A8 Alexa Fluor® 647
Pacific Blue™ anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 Pacific Blue™
APC anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 APC
PerCP/Cy5.5 anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 PerCP/Cy5.5
PE/Cy7 anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 PE/Cy7
Alexa Fluor® 700 anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 Alexa Fluor® 700
APC/Cy7 anti-mouse Ly-6G
C57BL/6 bone marrow cells stained with 1A8 APC/Cy7
Brilliant Violet 421™ anti-mouse Ly-6G
C57BL/6 mouse bone marrow cells were stained with Ly-6G (clone 1A8) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram). Data shown was gated on myeloid cell population.
Brilliant Violet 570™ anti-mouse Ly-6G
C57BL/6 bone marrow cells were stained with Ly-6G (clone 1A8) Brilliant Violet 570™ (filled histogram) or rat IgG2a, κ Brilliant Violet 570™ isotype control (open histogram). Data shown was gated on myeloid cell population.
