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PerCP/Cy5.5 anti-mouse IgM Antibody
PerCP/Cy5.5 anti-mouse IgM Antibody
406511 25 μg ¥25,000     
406512 100 μg ¥55,000     
Product Details
Clone: RMM-1
Isotype: Rat IgG2a, κ
Reactivity: Mouse
Immunogen: mouse IgGs and IgM, IgE, IgA cocktail
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested
Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
COA:
Enter Lot#:   
Application
Notes:

Each lot of this antibody is quality control tested by immunofluorescent staining and flow cytometric analysis.
  Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application
References:

1. Tertilt C, et al. 2009. Infect Immun. 77:3044. (ELISA) PubMed
2. Gibb DR, et al. 2011. J Immunol. 186:4244. PubMed
C57BL/6 splenocytes stained with RMM-1
C57BL/6 splenocytes stained with RMM-1 PerCP/Cy5.5


Compare all formats

See PerCP/Cy5.5 spectral data



Description:

The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells. The RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
Other Names: Immunoglobulin M
Structure: Ig family
Distribution: B cells
Function: B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
GeneID: 16019
Latest Publications: View the latest IgM articles on HighwirePress.com
UniProt: View information about IgM on UniProt.org
Keywords: PerCP/Cy5.5 anti-mouse IgM, RMM-1, PerCP/Cy5.5, Immunoglobulin M, Mouse, Immunology, Antibodies
Related Products
DescriptionCloneApplications
Cell Staining BufferFC, ICC, ICFC
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-mouse IgM
    C57BL/6 Mouse splenocytes stained with

    C57BL/6 Mouse splenocytes stained with purified RMM-1, followed by anti-rat IgG FITC

  • PE anti-mouse IgM
    C57BL/6 splenocytes stained with RMM-1

    C57BL/6 splenocytes stained with RMM-1 PE and 6D5 (CD19) APC

  • Biotin anti-mouse IgM
    C57BL/6 Mouse splenocytes stained with

    C57BL/6 Mouse splenocytes stained with biotinylated RMM-1, followed by Sav-PE

  • FITC anti-mouse IgM
    C57BL/6 splenocytes stained with RMM-1

    C57BL/6 splenocytes stained with RMM-1 FITC

  • APC anti-mouse IgM
    C57BL/6 splenocytes stained with RMM-1

    C57BL/6 splenocytes stained with RMM-1 APC

  • PerCP/Cy5.5 anti-mouse IgM
    C57BL/6 splenocytes stained with RMM-1

    C57BL/6 splenocytes stained with RMM-1 PerCP/Cy5.5

  • APC/Cy7 anti-mouse IgM
    C57BL/6 splenocytes stained with CD19

    C57BL/6 splenocytes stained with CD19 (6D5) FITC and RMM-1 APC/Cy7

    C57BL/6 splenocytes stained with CD19

    C57BL/6 splenocytes stained with CD19 (6D5) FITC and rat IgG2a APC/Cy7 isotype control

  • Brilliant Violet 421™ anti-mouse IgM
    C57BL/6 splenocytes were stained with

    C57BL/6 splenocytes were stained with CD19 FITC and anti-IgM (clone RMM-1) Brilliant Violet 421™ (top) or rat IgG2a Brilliant Violet 421™ isotype control (bottom).





  • PE/Cy7 anti-mouse IgM
    C57BL/6 splenocytes stained with CD19

    C57BL/6 splenocytes stained with CD19 (6D5) APC and RMM-1 PE/Cy7

    C57BL/6 splenocytes stained with CD19

    C57BL/6 splenocytes stained with CD19 (6D5) APC and rat IgG2a PE/Cy7 isotype control

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