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The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen tissue sections.
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. McNical AM, et al. 2007. Eur. J. Immunol. 37:1634.
C57BL/6 mouse splenocytes stained with YTS156 PerCP/Cy5.5
CD8b is the 32 kD β chain of CD8, also known as Lyt-3 or Ly-3. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation.
Other Names:
Lyt-3, Ly-3
Structure:
Ig superfamily, CD8β chain, 34 kD
Distribution:
majority of thymocytes, cytotoxic T cell subsets
Function:
Co-receptor of TCR
Ligand Receptor:
MHC class I
Antigen References:
1.Barclay A, et al. 1997. The Leukocyte antigen Facts Book Academic Press. 2. Zamoyska R. 1994. Immunity 1:243-246. 3. Ellmeier W, et al. 1999. Annu. Rev Immunol 17:523. 4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD8b
C57BL/6 mouse splenocytes stained with purified YTS156.7.7, followed by anti-rat IgG FITC
Biotin anti-mouse CD8b
BALB/c mouse splenocytes stained with biotinylated YTS156.7.7, followed by Sav-PE
FITC anti-mouse CD8b
BALB/c mouse splenocytes stained with YTS156.7.7 FITC
PE anti-mouse CD8b
C57BL/6 mouse splenocytes stained with YTS156.7.7 PE
PerCP/Cy5.5 anti-mouse CD8b
C57BL/6 mouse splenocytes stained with YTS156 PerCP/Cy5.5
Alexa Fluor® 647 anti-mouse CD8b
57BL/6 mouse splenocytes stained with YTS156.7.7 Alexa Fluor® 647
