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PerCP/Cy5.5 anti-mouse CD34 Antibody
PerCP/Cy5.5 anti-mouse CD34 Antibody
128607 25 µg $150.00     
128608 100 µg $325.00     
Product Details
Clone: HM34
Isotype: Armenian Hamster IgG
Isotype Control:PerCP/Cy5.5 Armenian Hamster IgG Isotype Ctrl
Reactivity: Mouse
Immunogen: Mouse CD34 transfected BHK cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximun emission of 690 nm.
COA:
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Application
Notes:

The HM34 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs.
  Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Bend.3 mouse endothelial cells stained
Bend.3 mouse endothelial cells stained with HM34 PerCP/Cy5.5


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Description:

CD34 is a highly glycosylated hematopoietic progenitor antigen.Two isoforms of CD34 have been reported. CD34 is expressed on hematopoietic progenitors, as well as endothelial cells, brain and testis. CD34 is thought to function as an adhesion molecule for attachment of stem cells to extracellular matrix or stromal cells.
Other Names: Mucosialin
Structure: Type I membrane protein, highly glycosylated, 75-120 kd, two isoforms reported.
Distribution: Hematopoietic progenitors, brain, testis, endothelial cells
Function: Possible adhesion molecules thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Present carbohydrate ligands to selectins.
Ligand Receptor: L-selectin, and others.
Antigen
References:

1. Garlanda C, et al. 1997. Eur J Cell Biol 73:368
2. Brown J, et al. 1991. Int Immunol 3:175
GeneID: 12490
Latest Publications: View the latest CD34 articles on HighwirePress.com
UniProt: View information about CD34 on UniProt.org
Keywords: PerCP/Cy5.5 anti-mouse CD34, HM34, PerCP/Cy5.5, Mucosialin, Mouse, Immunology, Antibodies
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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
  • Purified anti-mouse CD34
    NIH/3T3 cell line stained with

    NIH/3T3 cell line stained with purified HM34, followed by biotinylated anti-Armenian hamster IgG and Sav-PE

  • Biotin anti-mouse CD34
    NIH/3T3 cell line stained with

    NIH/3T3 cell line stained with HM34 biotin, followed by Sav-PE

  • Alexa Fluor® 647 anti-mouse CD34
    NIH/3T3 cell line stained with

    NIH/3T3 cell line stained with HM34 Alexa Fluor® 647

  • PerCP/Cy5.5 anti-mouse CD34
    Bend.3 mouse endothelial cells stained

    Bend.3 mouse endothelial cells stained with HM34 PerCP/Cy5.5

  • PE anti-mouse CD34
    Fibroblast NIH/3T3 cells stained with

    Fibroblast NIH/3T3 cells stained with HM34 PE

  • APC anti-mouse CD34
    Mouse fibroblast NIH/3T3 cells stained

    Mouse fibroblast NIH/3T3 cells stained with HM34 APC.

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