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PerCP/Cy5.5 anti-mouse CD3 Antibody
PerCP/Cy5.5 anti-mouse CD3 Antibody
100217 25 µg $100.00 Qty:
    
100218 100 µg $275.00 Qty:
    

Product Details

 
Clone: 17A2 (See other available formats)
Isotype: Rat IgG2b, κ
Isotype Control:PerCP/Cy5.5 Rat IgG2b, κ Isotype Ctrl
Reactivity: Mouse
Immunogen: γδTCR-positive T-T hybridoma D1
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application:

FC - Quality tested

Recommended Usage:

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Cy® and CyDye® are registered trademarks of GE Healthcare.

Excitation
Laser:
Blue Laser (488 nm)
COA:
Enter Lot#:   
Application
Notes:

The 17A2 antibody recognizes ε/γ (but not ε/δ) of the CD3 complex. The 17A2 antibody can induce T cell activation and has been reported to deplete CD3+ cells in vivo. Additional reported applications (for the relevant formats) include: immunoprecipitation1, complement-mediated cytotoxicity1,3, immunohistochemical staining of acetone-fixed frozen sections1,4, in vitro stimulation of T cells1 and depletion of CD3+ cells in vivo2. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100208). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100238) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application
References:

  Publication Library
PerCP/Cy5.5 anti-mouse CD3 Antibody 17A2 Image 1
C57BL/6 splenocytes stained with 17A2 PerCP/Cy5.5


Compare all formats

See PerCP/Cy5.5 spectral data



Cell Surface Immunofluorescence Staining Protocol




Description:

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Other Names: T cell antigen receptor complex, T3
Structure: Ig superfamily, CD3/TCR, 20 kD
Distribution: Thymocytes (differentiation dependent), mature T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand Receptor: Peptide antigen/MHC-complex
Antigen
References:

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

GeneID: 12502
Latest Publications: View the latest CD3 articles on HighwirePress.com
UniProt: View information about CD3 on UniProt.org
Keywords: PerCP/Cy5.5 anti-mouse CD3, 17A2, PerCP/Cy5.5, T cell antigen receptor complex, T3, Mouse, Flow Cytometry, Immunology, Antibodies


Product Reviews

  Title Rating Date
BioLegend 100217 (FC) /4 2012-12-22
BioLegend 100217 (FC) /4 2012-12-22
BioLegend 100218 (FC) /4 2012-08-19
BioLegend 100218 (FC) /4 2012-07-19
Review this product
See more reviews using the Reviews Library


Product FAQs

 
How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?
PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.
For a complete list of FAQs on all our products, visit our FAQ page.
 
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Other Formats

DescriptionCloneApplications
Alexa Fluor® 488 anti-mouse CD317A2FC, IF, IHC
Alexa Fluor® 594 anti-mouse CD317A2FC, IHC, IF
Alexa Fluor® 647 anti-mouse CD317A2FC, IF, IHC
Alexa Fluor® 700 anti-mouse CD317A2FC
APC anti-mouse CD317A2FC
APC/Cy7 anti-mouse CD317A2FC
Biotin anti-mouse CD317A2FC
Brilliant Violet 421™ anti-mouse CD317A2FC, IF
Brilliant Violet 510™ anti-mouse CD317A2FC
Brilliant Violet 570™ anti-mouse CD317A2FC
Brilliant Violet 605™ anti-mouse CD317A2FC
Brilliant Violet 650™ anti-mouse CD317A2FC
Brilliant Violet 711™ anti-mouse CD317A2FC
Brilliant Violet 785™ anti-mouse CD317A2FC
FITC anti-mouse CD317A2FC
LEAF™ Purified anti-mouse CD317A2FC, IHC, IP, Activ, CMCD, Depletion
Pacific Blue™ anti-mouse CD317A2FC
PE anti-mouse CD317A2FC
PE/Cy7 anti-mouse CD317A2FC
Purified anti-mouse CD317A2FC, IHC, IP
Ultra-LEAF™ Purified anti-mouse CD317A2FC, IHC, IP, Activ, CMCD, Depletion


Related Pathways

Pathway
CD28 Signaling in T-Helper Cell
CD4 and CD8 T-Cell Lineage
ERK Signaling
Gamma-Delta T cells
Immunologic Networks 2011
Innate Immunity
MDSC-induced Immune Suppression
NFAT Signaling and Lymphocyte Interactions
TH1 Pathway (Cellular Immune Response)
Th17 Biology
TH2 Pathway (Humoral Immune Response)


Related Categories

Category
Immunology > Mouse Immunology > NK/NKT Cells > CD3 > 17A2
Immunology > Mouse Immunology > CD and Related Molecules > CD3 > 17A2
Immunology > Mouse Immunology > Th17 > CD3 > 17A2
Immunology > Mouse Immunology > Costimulatory Molecules > CD3 > 17A2
Immunology > Mouse Immunology > T Cell Receptors (TCRs) > CD3
Immunology > Mouse Immunology > Stem Cells > CD3
Immunology > Mouse Immunology > T Regulatory Cells (TReg) > CD3

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

Version: 1 Revision Date: 2012-11-30
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Compare Data Across All Formats
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-mouse CD3
    C57BL/6 splenocytes stained with 17A2

    C57BL/6 splenocytes stained with 17A2 FITC and 145-2C11 PE

  • LEAF™ Purified anti-mouse CD3
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with LEAF™ purified 17A2, followed by anti-rat IgG FITC

  • PE anti-mouse CD3
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with 17A2 PE

  • Purified anti-mouse CD3
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with purified 17A2, followed by anti-rat IgG FITC

  • Alexa Fluor® 647 anti-mouse CD3
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 647

  • Alexa Fluor® 488 anti-mouse CD3
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 488

  • Pacific Blue™ anti-mouse CD3
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with Pacific Blue™ 17A2

  • Alexa Fluor® 700 anti-mouse CD3
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with 17A2 Alexa Fluor® 700

  • PerCP/Cy5.5 anti-mouse CD3
    C57BL/6 splenocytes stained with 17A2

    C57BL/6 splenocytes stained with 17A2 PerCP/Cy5.5

  • PE/Cy7 anti-mouse CD3
    C57BL/6 splenocytes stained with 17A2

    C57BL/6 splenocytes stained with 17A2 PE/Cy7

  • APC/Cy7 anti-mouse CD3
    C57BL/6 splenocytes stained with 17A2

    C57BL/6 splenocytes stained with 17A2 APC/Cy7

  • Brilliant Violet 421™ anti-mouse CD3
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 421™ (top) or rat IgG2b, κ Brilliant Violet 421™ isotype control (bottom).





    C57BL/6 mouse splenocytes were fixed

    C57BL/6 mouse splenocytes were fixed with 2% paraformaldehyde (PFA), and then stained with 5 µg/ml CD3 (clone 17A2) Brilliant Violet 421™ (blue) and 5 µg/ml CD19 (clone 6D5) Alexa Fluor® 647 (red) for 30 minutes at room temperature. The image was captured by 40X objective.

  • Brilliant Violet 570™ anti-mouse CD3
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2 Brilliant Violet 570™ (top) or rat IgG2b, κ Brilliant Violet 570™ isotype control (bottom).





  • Brilliant Violet 650™ anti-mouse CD3
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 650™.

  • Brilliant Violet 785™ anti-mouse CD3
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 785™.

  • Brilliant Violet 510™ anti-mouse CD3
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2) Brilliant Violet 510™.

  • APC anti-mouse CD3
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2) APC (filled histogram) or rat IgG2b, κ isotype control (open histogram).

  • Ultra-LEAF™ Purified anti-mouse CD3
    C57BL/6 mouse splenocytes stained with

    C57BL/6 mouse splenocytes stained with Ultra-LEAF™ purified CD3 (clone 17A2), followed by anti-rat IgG FITC.

  • Brilliant Violet 605™ anti-mouse CD3
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 605™.

  • Alexa Fluor® 594 anti-mouse CD3
    C57BL/6 mouse splenocytes were stained

    C57BL/6 mouse splenocytes were stained with CD3 (clone 17A2) Alexa Fluor® 594 (filled histogram). The data was acquired by BD LSRFortessa™ cell analyzer equipped with Yellow-Green Laser (561 nm).

    C57BL/6 mouse frozen spleen section

    C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS plus 5% rat serum for 1 hour at room temperature. Then the section was stained with 2.5 µg/ml of CD3 (clone 17A2) Alexa Fluor® 594 (red) and 2.5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (green) overnight at 4°C. The image was captured by 10X objective.

  • Brilliant Violet 711™ anti-mouse CD3
    C57BL/6 splenocytes were stained with

    C57BL/6 splenocytes were stained with CD19 APC and CD3 (clone 17A2) Brilliant Violet 711™ (top) or rat IgG2b, κ Brilliant Violet 711™ isotype control (bottom).





  • Biotin anti-mouse CD3
    C57BL/6 splenocytes were stained with

    C57BL/6 splenocytes were stained with CD19 APC and biotinylated CD3 (clone 17A2) (top) or biotinylated rat IgG2b, κ isotype control (bottom), followed by SAV-PE.





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