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The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 102112). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102116) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim) 2. Cibotti R, et al. 1997. Immunity 6:245. (Costim) 3. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block) 4. Nishio M, et al. 1996. J. Immunol. 157:4347. (Block) 5. Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim) 6. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim) 7. Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim) 8. Wang W, et al. 2007. J. Immunol. 178:4885. (Costim) 9. Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim) 10. Perchonock CE, et al. 2007. J. Immunol. 179:1768. 11. Barbi J, et al. 2007. Blood 110:2215. 12. Milpied P, et al. 2011. Blood 118:2993. PubMed 13. Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed
C57BL/6 mouse splenocytes were stained with CD3 FITC and CD28 (clone 37.51) PerCP/5.5 (top) or Syrian hamster IgG PerCP/5.5 isotype control (bottom).
CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.
Other Names:
Tp44, T44
Structure:
Ig superfamily, 44 kD
Distribution:
Thymocytes, CD4+, CD8+ peripheral T cells, NK cells
Function:
Costimulates T and NK cells
Ligand Receptor:
CD80 (B7-1), CD86 (B7-2)
Antigen References:
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233. 3. Gross JA, et al. 1992. J. Immunol. 149:380.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD28
C57BL/6 mouse splenocytes were stained with CD3 FITC and CD28 (clone 37.51) APC (top) or Syrian hamster IgG APC isotype control (bottom).
Biotin anti-mouse CD28
C57BL/6 mouse splenocytes were stained with CD3 APC and Biotinylated CD28 (clone 37.51) (top) or Syrian Hamster IgG isotype control (bottom), followed by Sav-PE (bottom).
LEAF™ Purified anti-mouse CD28
C57BL/6 splenocytes stained with LEAF™ purified 37.51, followed by anti-Syrian hamster IgG FITC
PE anti-mouse CD28
C57BL/6 splenocytes stained with
37.51 PE and CD3 FITC
PE/Cy5 anti-mouse CD28
C57BL/6 splenocytes stained with
37.51 PE and CD3 FITC
C57BL/6 mouse splenocytes stained with CD3 (145-2C11) FITC and 37.51 PE/Cy5
Purified anti-mouse CD28
C57BL/6 splenocytes stained with
37.51 PE and CD3 FITC
PerCP/Cy5.5 anti-mouse CD28
C57BL/6 mouse splenocytes were stained with CD3 FITC and CD28 (clone 37.51) PerCP/5.5 (top) or Syrian hamster IgG PerCP/5.5 isotype control (bottom).
Ultra-LEAF™ Purified anti-mouse CD28
C57BL/6 splenocytes stained with LEAF™ purified 37.51, followed by anti-Syrian hamster IgG FITC
