Welcome to BioLegend’s one-of-a-kind advanced search. Search by any one of the single fields below or narrow down your searches by using multiple parameters. Looking for an anti-mouse NK cell antibody for IHC? Or looking for a FITC-conjugated antibody against a human marker for dendritic cells to use for flow cytometry? Only BioLegend’s advanced search can provide you with the best answers. If you have further questions, please contact our technical service team at 858-768-5801.
Use this document lookup tool to find Current Product Datasheets, Certificates of Analysis, or MSDS for any antibody products you have purchased from BioLegend.
Use the search box below to perform a site search of BioLegend.com powered by Google™.
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
COA:
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
CD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.
Other Names:
CR2/CR1
Structure:
145 kD/190 kD type I transmembrane glycoprotein
Distribution:
B cells, follicular dendritic cells
Function:
Signal transduction
Ligand Receptor:
Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
Antigen References:
1. Kozono Y, et al. 1998. J. Immunol. 160:1562. 2. Shimizu I, et al. 2007. Blood 109:1773. 3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
PE/Cy7 anti-mouse CD21/CD35 (CR2/CR1)
C57BL/6 mouse splenocytes stained with 7E9 PE/Cy7
APC/Cy7 anti-mouse CD21/CD35 (CR2/CR1)
C57BL/6 mouse splenocytes stained with 7E9 APC/Cy7 and CD3 PE
Purified anti-mouse CD21/CD35 (CR2/CR1)
C57BL/6 mouse splenocytes stained with purified 7E9, followed by anti-rat IgG FITC
LEAF™ Purified anti-mouse CD21/CD35 (CR2/CR1)
C57BL/6 mouse splenocytes stained with LEAF™ purified 7E9, followed by anti-rat IgG FITC
Biotin anti-mouse CD21/CD35 (CR2/CR1)
C57BL/6 mouse splenocytes stained with biotinylated 7E9, followed by Sav-PE
FITC anti-mouse CD21/CD35 (CR2/CR1)
C57BL/6 mouse splenocytes stained with 7E9 FITC
PE anti-mouse CD21/CD35 (CR2/CR1)
C57BL/6 mouse splenocytes stained with 7E9 PE
APC anti-mouse CD21/CD35 (CR2/CR1)
C57BL/6 mouse splenocytes stained with 7E9 APC
Pacific Blue™ anti-mouse CD21/CD35 (CR2/CR1)
C57BL/6 splenocytes stained with 7E9 Pacific Blue™
