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The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. * PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
COA:
Application Notes:
The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm sterile-filtered) is recommended for functional assays (Cat. No. 126517). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 126526) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Krug A, et al. 2002. J. Immunol. 169:6079. 2. McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed 3. Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed 4. Barbi J, et al. 2009. FASEB J. 23:3990. PubMed 5. Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed 6. Dürr C, et al. 2010. Cancer Res. 70:10170. (FA) PubMed
CD183, also known as CXCR3, is a member of the C-X-C chemokine family, characterized by a pair of cysteine residues separated by a single amino acid. CXCR3 is a 38 kD seven pass transmembrane receptor coupled to G-protein. It mediates Ca2+ mobilibization and chemotaxis in response to C-X-C chemokines, such as IP10 (CXCL10), MIG (CXCL9), I-TAC (CXCL11) and PF4 (CXCL4). CXCR3 is expressed primarily on activiated T lymphocytes, NK cells, and some epithelial cells and endothelial cells. It is not expressed on B cells, monocytes or granulocytes.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
LEAF™ Purified anti-mouse CD183 (CXCR3)
C57BL/6 splenocytes stained with purified CXCR3-173, followed by anti-Armenian hamster IgG FITC and anti-mouse CD3 (17A2) APC
PE anti-mouse CD183 (CXCR3)
C57BL/6 splenocytes stained with CXCR3-173 PE and B220 FITC
Biotin anti-mouse CD183 (CXCR3)
C57BL/6 splenocytes stained with B220 (RA3-6B2) FITC and CXCR3-173 Biotin, followed by SAV-PE
Purified anti-mouse CD183 (CXCR3)
C57BL/6 splenocytes stained with purified CXCR3-173, followed by anti-Armenian hamster IgG FITC and anti-mouse CD3 (17A2) APC
APC anti-mouse CD183 (CXCR3)
C57BL/6 splenocytes stained with B220 (RA3-6B2) FITC and CXCR3-173 APC
C57BL/6 splenocytes stained with CXCR3-173 PE/Cy7 and CD3e (145-2C11) APC
C57BL/6 splenocytes stained with Armenian Hamster IgG isotype PE/Cy7 and CD3e (145-2C11) APC
Brilliant Violet 421™ anti-mouse CD183 (CXCR3)
C57BL/6 mouse splenocytes were stained with CD3 FITC and CXCR3 (clone CXCR3-173) Brilliant Violet 421™ (top) or Armenian hamster IgG Brilliant Violet 421™ isotype control (bottom).
Ultra-LEAF™ Purified anti-mouse CD183 (CXCR3)
C57BL/6 splenocytes stained with purified CXCR3-173, followed by anti-Armenian hamster IgG FITC and anti-mouse CD3 (17A2) APC
