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The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Concentration:
0.2 mg/ml
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
COA:
Application Notes:
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen and formalin-fixed paraffin-embedded tissue sections.
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. 2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London. 3. Yang M, et al. 2012. J Appl Physiol. 112:204. PubMed.
Human peripheral blood granulocytes stained with G10F5 PerCP/Cy5.5
CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6 and NCA-95. CD66b is a member of the immunoglobulin superfamily and carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.
Other Names:
CD67, CGM6, NCA-95
Structure:
Ig superfamily, CEA antigen group, GPI-linked glycoprotein, 95-100 kD
Distribution:
Granulocytes
Function:
Cell adhesion, neutrophil activation
Ligand Receptor:
CD66c
Antigen References:
1. Kuijpers T, et al. 1993. J. Immunol. 151:4934. 2. Kuroki M, et al. 1992. J. Leuk. Biol. 52:551.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD66b
Human peripheral whole blood granulocytes stained with G10F5 FITC
Purified anti-human CD66b
Human peripheral blood granulocytes stained with purified G10F5, followed by anti-mouse Igs FITC
PE anti-human CD66b
Human peripheral blood granulocytes stained with G10F5 PE
PerCP/Cy5.5 anti-human CD66b
Human peripheral blood granulocytes stained with G10F5 PerCP/Cy5.5
