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The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximun emission of 690 nm.
COA:
Application Notes:
Additional reported applications inclu de: immunoprecipitation (1), inhibit the interaction of ICAM-3 and LFA-1(1)
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. deFougerolles AR. and TA. Springer et al. 1992. J. Exp. Med. 175:185 Becton Dickinson and Co. ("BD") is the owner of European Patent No. EP 0 314 406 which still is in force in Germany (DE3883478) France Italy and the United Kingdom and Japanese Patent No. JP1888759. These patents cover any composition consisting of PerCP (peridinin-chlorophyll-protein-complex) attached to a member of a specific binding pair such as a PerCP-labeled antibody and the use thereof. PerCP-labeled antibodies are not available for sale in above mentioned countries. Please inquire regarding the expected availability date of such products.
Human peripheral blood lymphocytes stained with CBR-IC3/1 PerCP/Cy5.5
CD50 is a 120-140 kD type I membrane protein also known as intercellular adhesion molecule 3 (ICAM-3). It is broadly expressed on leukocytes, endothelial cells, and Langerhans cells. CD50 is a member of the Ig superfamily. It provides costimulatory signal for T cells and regulates cell adhesion through interaction with LFA-1 (CD11a/CD18, αL/β2) integrin.
Other Names:
ICAM-3
Structure:
Ig superfamily, type I membrane protein, 130 kD
Distribution:
Thymocytes, T and B cells, monocytes, granulocytes, endothelial cells
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD50 (ICAM-3)
Human peripheral blood lymphocytes stained with purified CBR-IC3/1, followed by anti-mouse IgG FITC
PE anti-human CD50 (ICAM-3)
Human peripheral blood lymphocytes stained with CBR-IC3/1 PE
Alexa Fluor® 647 anti-human CD50 (ICAM-3)
Human peripheral blood lymphocytes stained with CBR-IC3/1 Alexa Fluor®647
APC anti-human CD50 (ICAM-3)
Human peripheral blood lymphocytes stained with CBR-IC3/1 APC
PerCP/Cy5.5 anti-human CD50 (ICAM-3)
Human peripheral blood lymphocytes stained with CBR-IC3/1 PerCP/Cy5.5
