The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximum emission of 690 nm.
Application References:
1. Poggi, A., et al. 1995. Eur. J. Immunol. 25: 369
Human peripheral blood lymphocytes stained with NKTA255 PerCP/Cy5.5 and anti-CD19 (HIB19) APC
Description:
CD305, also known as LAIR-1 (leukocyte-associated Ig-like receptor-1), is a 40 kD type I transmembrane glycoprotein. LAIR-1 and LAIR-2 are the only members of the LAIR family in the Ig superfamily. LAIR-1 is an inhibitory molecule characterized by ITIMs in the cytoplasmic domain and collagen ligands. CD305 is expressed on NK cells, T cells, B cells, monocytes, dendritic cells, eosinophils, basophils and mast cells. LAIR-1 functions in the inhibition of cell cytotoxicity, cell activation, proliferation and differentiation.
Other Names:
LAIR-1 (Leukocyte-associated Ig-like receptor 1)
Structure:
40 kD type I transmembrane protein, cytoplasmic domain contains ITIM motifs, Ig superfamily
Distribution:
NK cells, T cell, B cells, monocytes, dendritic cells, eosinophils, basophils and mast cells
Function:
Inhibition of cell cytotoxicity, cell activation, proliferation and differentiation
Ligand Receptor:
collagens
Antigen References:
1. Meyaard, L., et al., 2008. J. Leukoc. Biol. 83: 799 2. Lebbink, RJ., et al. 2006. J. Exp. Med. 203: 1419
