The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Application References:
1. Poggi, A., et al. 1995. Eur. J. Immunol. 25: 369
Human peripheral blood lymphocytes stained with NKTA255 PerCP/Cy5.5 and anti-CD19 (HIB19) APC
Description:
CD305, also known as LAIR-1 (leukocyte-associated Ig-like receptor-1), is a 40 kD type I transmembrane glycoprotein. LAIR-1 and LAIR-2 are the only members of the LAIR family in the Ig superfamily. LAIR-1 is an inhibitory molecule characterized by ITIMs in the cytoplasmic domain and collagen ligands. CD305 is expressed on NK cells, T cells, B cells, monocytes, dendritic cells, eosinophils, basophils and mast cells. LAIR-1 functions in the inhibition of cell cytotoxicity, cell activation, proliferation and differentiation.
Other Names:
LAIR-1 (Leukocyte-associated Ig-like receptor 1)
Structure:
40 kD type I transmembrane protein, cytoplasmic domain contains ITIM motifs, Ig superfamily
Distribution:
NK cells, T cell, B cells, monocytes, dendritic cells, eosinophils, basophils and mast cells
Function:
Inhibition of cell cytotoxicity, cell activation, proliferation and differentiation
Ligand Receptor:
collagens
Antigen References:
1. Meyaard, L., et al., 2008. J. Leukoc. Biol. 83: 799 2. Lebbink, RJ., et al. 2006. J. Exp. Med. 203: 1419
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