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The antibody was purified by affinity chromatography and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
COA:
Application Notes:
Additional reported applications include: inducing cytokine production by NK cells, enhancing NK cytotoxicity, and Western blotting.
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Valiante NM and Trinchieri G. 1993. J. Exp. Med. 178:1397. 2. Chuang SS, et al. 2000. Immunology. 100:378. 3. Hoffmann SC, et al. 2011. J. Immunol. 186:2757. PubMed
Human peripheral lymphocytes were stained with CD8 FITC, and CD244 (clone C1.7) PerCP/Cy5.5 (top) or mouse IgG1, κ PerCP/Cy5.5 isotype control (bottom).
CD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including majority of CD8+ T cells and γ/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.
Other Names:
2B4
Structure:
38 kD type I transmembrane protein, CD2 family member, Ig superfamily
Distribution:
NK, T subset, monocytes, basophils, and eosinophils
Function:
Activation and inhibition of NK cell function
Ligand Receptor:
CD48
Antigen References:
1. Lee KM, et al. 2003. J. Immunol. 170:4881. 2. Chlewicki LK, et al. 2008. J. Immunol. 180:8159. 3. Messmer B, et al. 2006. J. Immunol. 176:4646. 4. Boles KS, et al. 2001. Immunol. Rev. 181:234.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD244 (2B4)
Human peripheral blood lymphocytes stained with purified C1.7, followed by anti-mouse IgG FITC
FITC anti-human CD244 (2B4)
Human peripheral blood lymphocytes stained with CD8 (RPA-T8) APC and C1.7 FITC
PE anti-human CD244 (2B4)
Human peripheral blood lymphocytes stained with C1.7 PE
APC anti-human CD244 (2B4)
Human peripheral blood lymphocytes stained with C1.7 APC
PerCP/Cy5.5 anti-human CD244 (2B4)
Human peripheral lymphocytes were stained with CD8 FITC, and CD244 (clone C1.7) PerCP/Cy5.5 (top) or mouse IgG1, κ PerCP/Cy5.5 isotype control (bottom).
