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The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
COA:
Cy3, Cy5, Cy5.5 and Cy7 are subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under license from GE Healthcare Bio-Sciences Corp. Sale of this product is licensed for research use only.
Application References:
1. Guo M, et al. 2000. Human Immunology 61:729. 2. Cho H, et al. 2007. Physiol Genomics doi:10.1152/physiolgenomics.00051.2006 3. Gurer C, et al. 2008. Blood 112:1231. PubMed
Human monocyte-derived dendritic cells stained with HD30 PerCP/Cy5.5
CD205 is a 210 kD C-type lectin transmembrane protein, known as DEC-205. It belongs to macrophage mannose receptor family and is found at high levels on dendritic cells and thymic epithelial cells. Unlike murine CD205, human CD205 is also expressed at low levels on T- and B-cells, NK cells and monocytes. CD205, serves as an endocytic receptor, functions in antigen uptake/processing and clearance of apoptotic cells.
High levels on dendritic cells and thymic epithelial cells, low levels on lymphocytes and monocytes
Function:
Antigen uptake and process
Antigen References:
1. Guo M, et al. 2000. Human Immunology 61:729. 2. Kato M, et al. 2006. Int. Immunol. 3. Kato M, et al. 2003. J. Biol. Chem. 278(36):34035. 4. Small M and G. Kraal. 2003. Int. Immunol. 15(2):197. 5. Mahnke K, et al. 2000. J. Cell Biol. 151(3):673.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/terms). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.
This data display is provided for general comparisons between formats. Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors. If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-human CD205 (DEC-205)
Monocyte-derived dendritic cells stained with HD30 PE
PE anti-human CD205 (DEC-205)
Monocyte-derived dendritic cells stained with HD30 PE
Alexa Fluor® 647 anti-human CD205 (DEC-205)
Monocyte-derived dendritic cells stained with HD30 Alexa Fluor® 647
APC anti-human CD205 (DEC-205)
Human monocyte-derived dendritic cells stained with HD30 APC
PerCP/Cy5.5 anti-human CD205 (DEC-205)
Human monocyte-derived dendritic cells stained with HD30 PerCP/Cy5.5
