The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Application:
FC - Quality tested
Recommended Usage:
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and 564 nm and a maximum emission of 690 nm.
Human peripheral blood lymphocytes stained with CD19 (HIB19) PE and TG7/CCR6 PerCP/Cy5.5
Description:
CCR6 is a chemokine receptor that is expressed on immature dendritic cells, Blymphocytes, and memory T cells (1). CCR6 bindsCCL20, although members of the β defensin family also bind CCR6 with a lower affinity (2). CCR6 positive cells and its ligand CCL20 have been detected in numerous organs, especially the secondarylymphoid organ. CCL20 is selectively made by the follicle-associated epithelium (FAE) overlying Peyer's patches (PPs) and isolated lymphoid follicles (ILFs). CCL20 contributes to the recruitment of CCR6-expressing B cells to these structures (3). In humans, CCR6can function to mediate arrest of T cells on dermal endothelialcells and is highly expressed on T cells resident in bothnormal and psoriatic skin (4), and CCR6 and/or CCL20have been implicated in the pathogenesis of rheumatoid arthritis(5) and inflammatory bowel disease. Human T cells that are able to produce IL-17 express CCR6. It suggests that CCL20 and CCR6 havea role in inflammatory diseases by recruiting Th17 cells to targettissues (6).
CCR6 is expressed in immature dendritic cells, B lymphocytes, and memory T cells, Th17 cells.
Function:
CCR6 has been reported to have roles in host defense and inflammation particularly at epithelial sites. CCR6 and CCL20 are important for recruitment of B cells to secondary lymphoid organs. In addition, CCR6 mediate arrest of T cells on dermal endothelial cells.
Ligand Receptor:
CCL20 (MIP-3α)
Antigen References:
1. Zaballos A, et al., Biochem Biophys Res Commun, 227:846-853, 1996. 2. Yang D., et al., Science, 286:525-528, 1999. 3. MacDonald KG, et al., Am J of Path, 170:1229-1240, 2007. 4. Homey B, et al., J Immun, 164: 6621-6632, 2000. 5. Hirota K, et al., J Exp Med, 204:2803-2812, 2007. 6. Singh SP, et al., J Immun, 180: 214-221, 2008.
