The antibody was purified by affinity chromatography, and conjugated with PerCP/Cy5.5 under optimal conditions. The solution is free of unconjugated PerCP/Cy5.5 and unconjugated antibody.
Storage & Handling:
The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is 5 µl per million cells or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cy5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Human peripheral blood lymphocytes stained with CD19 (HIB19) PE and TG7/CCR6 PerCP/Cy5.5
Description:
CCR6 is a chemokine receptor that is expressed on immature dendritic cells, Blymphocytes, and memory T cells (1). CCR6 bindsCCL20, although members of the β defensin family also bind CCR6 with a lower affinity (2). CCR6 positive cells and its ligand CCL20 have been detected in numerous organs, especially the secondarylymphoid organ. CCL20 is selectively made by the follicle-associated epithelium (FAE) overlying Peyer's patches (PPs) and isolated lymphoid follicles (ILFs). CCL20 contributes to the recruitment of CCR6-expressing B cells to these structures (3). In humans, CCR6can function to mediate arrest of T cells on dermal endothelialcells and is highly expressed on T cells resident in bothnormal and psoriatic skin (4), and CCR6 and/or CCL20have been implicated in the pathogenesis of rheumatoid arthritis(5) and inflammatory bowel disease. Human T cells that are able to produce IL-17 express CCR6. It suggests that CCL20 and CCR6 havea role in inflammatory diseases by recruiting Th17 cells to targettissues (6).
CCR6 is expressed in immature dendritic cells, B lymphocytes, and memory T cells, Th17 cells.
Function:
CCR6 has been reported to have roles in host defense and inflammation particularly at epithelial sites. CCR6 and CCL20 are important for recruitment of B cells to secondary lymphoid organs. In addition, CCR6 mediate arrest of T cells on dermal endothelial cells.
Ligand Receptor:
CCL20 (MIP-3α)
Antigen References:
1. Zaballos A, et al., Biochem Biophys Res Commun, 227:846-853, 1996. 2. Yang D., et al., Science, 286:525-528, 1999. 3. MacDonald KG, et al., Am J of Path, 170:1229-1240, 2007. 4. Homey B, et al., J Immun, 164: 6621-6632, 2000. 5. Hirota K, et al., J Exp Med, 204:2803-2812, 2007. 6. Singh SP, et al., J Immun, 180: 214-221, 2008.
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